The identification of long-noncoding RNAs associated with the formation of atherosclerotic plaques and their therapeutic manipulation strategy
| Project/Area Number |
25670028
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Osaka University |
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Principal Investigator |
OBIKA Satoshi 大阪大学, 薬学研究科(研究院), 教授 (80243252)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Tsuyoshi 大阪大学, 薬学研究科(研究院), 助教 (80636994)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ノンコーディングRNA / 核酸医薬 / 動脈硬化 / マクロファージ / 長鎖ノンコーディングRNA / 動脈硬化症 / アンチセンス医薬 / 分化 |
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| Outline of Final Research Achievements |
It has not yet been established a therapeutic strategy to reduce atherosclerotic lesions and plaques. We here tried to identify a novel long-noncoding RNA (lncRNA) associated with plaque formation and progression. We initially established an isolation methodology of monocytes from mice and handling of differentiated polarized macrophages. From these isolated primary cells, we prepared complementary DNAs and analyzed up- or down-regulated lncRNAs. We observed significant changes in expression for some lncRNAs upon polarization. We further invested whether oligonucleotide-based therapeutics can be a potential therapeutic strategy for manipulating lncRNA function. These findings would further enable us to develop a therapeutic strategy to treat atherosclerosis.
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Report
(3 results)
Research Products
(3 results)
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[Journal Article] Amido-bridged nucleic acids with small hydrophobic residues enhance hepatic tropism of antisense oligonucleotides in vivo.2015
Author(s)
１．Yamamoto, T., Yahara, A., Waki, R., Yasuhara, H., Wada, F., Harada-Shiba, M. and Obika, S.
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Journal Title
Org Biomol Chem.
Volume: 13
Issue: 12
Pages: 3757-3765
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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