Study on the molecular mechanisms for interaction between mesenchymal stem cells and blood brain barrier using real-time imaging
| Project/Area Number |
25670033
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Pharmacology in pharmacy
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
MINAMI Masabumi 北海道大学, 薬学研究科(研究院), 教授 (20243040)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ISO Yukina
MATSUBARA Akira
MINAMISHIMA Takuya
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | 神経科学 / 移植・再生医療 / 薬学 / 血液脳関門 / 骨髄幹細胞 / リアルタイムイメージング |
|---|
| Outline of Final Research Achievements |
We carried out antibody array analysis to identify the proteins contained in mouse astrocyte-conditioned medium (ACM), and examined the effects of those proteins on the blood-brain barrier (BBB) function using a mouse in vitro BBB model. We found that leukemia inhibitory facto (LIF) was contained in ACM and significantly attenuated barrier function. Because LIF belongs to Interleukin (IL)-6 family, we examined the effect of other family members IL-6 and oncostatin M on BBB function. Oncostatin M, but not IL-6, significantly attenuated barrier function. Further study for the molecular mechanism of the effects of LIF and oncostatin M may lead to development of the method to control BBB function.
|
Report
(3 results)
Research Products
(3 results)
