Neural stem cells have directivity characteristics in the cell fate decision.

• Project/Area Number: 25670037
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pharmacology in pharmacy
• Research Institution: Osaka University
• Principal Investigator: KASAI Atsushi 大阪大学, 薬学研究科(研究院), 助教 (40454649)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: miRNA / 神経幹細胞 / セロトニン / noggin / 運命決定 / BMP

Outline of Final Research Achievements

Neural stem cells have pluripotency to differentiate into a variety of neural cells, such as neuron and glia cells. Here, we showed that noggin treatment to embryonic stem cells changed miRNA expression patterns in neural stem cells differentiated by diverse methods and increased serotonin neuronal differentiation. These findings suggest that pluripotency of neural stem cells may be already assigned the directional differentiation.

Research Products

• [Journal Article] Identification of the role of BMP and TGF-β signaling in the trajectory of serotonergic differentiation in a rapid assay in mouse embryonic stem cells in vitro. (2015)
  • Author(s): Yamasaki A, Kasai A, Toi A, Kurita M, Kimoto S, Hayata-Takano A, Nakazawa T, Nagayasu K, Shintani N, Hashimoto R, Ito A, Meltzer HY, Ago Y, Waschek JA, Onaka Y, matsuda T, Baba A, Hashimoto H.
  • Journal Title: Journal of Neurochemistry, Volume: 132(4) Issue: 4 Pages: 418-428
  • DOI: 10.1111/jnc.12999
  • Peer Reviewed / Open Access
• [Presentation] LPS誘発炎症モデルにおいて発現変化する脳内miRNAの同定 (2014)
  • Author(s): 柿原素楽
  • Organizer: 第126回 日本薬理学会 近畿部会
  • Place of Presentation: 和歌山県JAビル
  • Year and Date: 2014-10-24
• [Presentation] 脳内miRNA発現の高感度検出に向けたin situ hybridization法の最適化：炎症により発現変化する脳内miRNAの同定 (2014)
  • Author(s): 柿原素楽
  • Organizer: 生体機能と創薬シンポジウム2014
  • Place of Presentation: 近畿大学東大阪キャンパス(大阪)
  • Year and Date: 2014-08-28 – 2014-08-29
• [Presentation] The balance between BMP and TGF-β signaling at an early stage steers serotonergic differentiation from ES cells (2014)
  • Author(s): Atsushi Yamasaki
  • Organizer: 第87回日本薬理学会年会
  • Place of Presentation: 仙台国際センター
• [Presentation] Roe of TGF-beta and BMP signaling in the trajectory of serotonergic differentiation from ES cells (2013)
  • Author(s): Atsushi Yamasaki
  • Organizer: Neuroscience 2013
  • Place of Presentation: San Diego, 米国
• [Remarks] 大阪大学大学院薬学研究科 神経薬理学分野
  • URL: http://molpharm.umin.jp/