| Project/Area Number |
25670045
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Natural medicines
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| Research Institution | Chiba University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | シグナル伝達 / 生理活性 / 薬学 / 生体分子 / 有機化学 / 蛋白質 |
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| Outline of Final Research Achievements |
This study aims at search for bioactive small molecule from natural resources that have effects on BMI1, which is a key regulatory component of the polycomb repressive complex1 (PRC1) as well as those that act on Wnt and hedgehog signaling pathways, which are known to control the expression of BMI1 molecule. First a cell-based luciferase assay system which assesses the BMI1 promoter activity was constructed, and screening study using this assay system afforded several plant extracts as hit samples. On the other hand, a diterpene from a Plantaginaceous plant and a limonoid from a Meliaceous plant with Wnt signal inhibitory activity were isolated, while a highly oxygenated steroid derivative with hedgehog inhibitory activity was obtained from Solanaceous plant.
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