Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Outline of Final Research Achievements |
To utilize high productivity of secondary metabolites of fungi, construction of benzyl isoquinoline alkaloids biosynthesis system in Aspergillus oryzae was attempted. Expression plasmids of tyrsinase, monoamine oxidase, tyrosine decarboxylase, and norcoclaurine synthase genes were constructed. These plasmids were introduced into the host fungus A. oryzae by transformation. Analysis of induction culture products of transformants did not give any detectable benzyl isoquinoline alkaloids. Thus the direction of study was changed to isolate endophyte fungi producing alkaloids from alkaloid producing plants. So far some endophyte fungi were obtained from Amaryllidaceae plants.
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