| Project/Area Number |
25670065
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental and hygienic pharmacy
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
HANADA KENTARO 国立感染症研究所, 細胞化学部, 部長 (30192701)
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| Co-Investigator(Kenkyū-buntansha) |
KUMAGAI Keigo 国立感染症研究所, 細胞化学部, 主任研究官 (40443105)
YAMAJI Toshiyuki 国立感染症研究所, 細胞化学部, 主任研究官 (50332309)
SAITO Kyoko 国立感染症研究所, 細胞化学部, 主任研究官 (70235034)
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| Co-Investigator(Renkei-kenkyūsha) |
SUGIKI Toshihiko 大阪大学, 蛋白質研究所, 助教 (70635698)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 脂質 / 輸送 / クラミジア / セラミド / プレクストリン相同ドメイン / ホスホイノシチド / 感染症 / 細胞内寄生 / Vero細胞 / ワクチン細胞基材 / ゲノム / 性感染症 / クラミジア菌 / 脂質輸送 / ゲノム編集 / プレスクトリン相同ドメイン / HeLa細胞 |
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| Outline of Final Research Achievements |
The obligate intracellular bacterium Chlamydia requires the ceramide transport protein CERT of host cells for infection, and the pleckstrin homology (PH) domain of CERT associates with the inclusion membrane protein IncD. The PH domain of CERT has a phosphatidylinositol 4-monophosphate (PI4P) binding activity to transport ceramide to the Golgi apparatus. We here showed that mutant PH domains defective in PI4P-binding are still capable of associating with IncD, suggesting an interaction of the PH domain with IncD at the region different from its PI4P-binding pocket. This study presents a molecular strategy for the prevention of Chlamydia hijacking of host cell CERT.
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