| Project/Area Number |
25670074
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical pharmacy
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
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| Research Collaborator |
UESHIMA Satoshi 立命館大学, 薬学部, 助教
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 薬物動態学 / 薬学 / 腎排泄 / トランスポーター / 培養細胞 |
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| Outline of Final Research Achievements |
To develop in vitro experimental model systems to predict the renal secretion of drugs in human kidney, we treated the human kidney-derived cultured cells by various reagents to stimulate the expression of drug transporters. When HK-2 cells were treated with DNA methyltransferase inhibitor, the expression of OCT2 mRNA was significantly enhanced. It was demonstrated that the transport function of OCT2 was also enhanced. Development of in vitro experimental model systems to predict the renal secretion of drugs in human kidney will be useful for drug discovery and development.
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