| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Fourteen CYPs which are drug metabolizing enzymes in human liver were reacted to more than eight kinds of adrenal steroids and their metabolites were identified. As a result, only CYP3A4 showed a various metabolite on HPLC chart. Production of androstendion from 17alpha-hydroxyprogesterone and 11-deoxycortisol by CYP3A4 was confirmed by GC-MS. It was revealed that CYP3A4 can catalyze side chain cleavage reaction (Lyase activity) to the steroids. 6beta-Hydoxy metabolite which were main metabolite from 11-deoxycortisol was identified. Then, Km and Vmax of 6beta-hydroxylase activity and Lyase activity against 11-deoxycortisol were determined and compared.
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