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Development of noninvasive drug metabolizing enzyme activity estimate method for side effect reduction of the drugs

Research Project

Project/Area Number 25670077
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Medical pharmacy
Research InstitutionOsaka Kyoiku University

Principal Investigator

Katagiri Masanao  大阪教育大学, 教育学部, 教授 (00185802)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsステロイド代謝 / 薬物代謝 / CYP3A4 / リアーゼ活性 / ヒト・CYP / 薬物代謝酵素 / 水酸化活性 / CYP / コルチソール / リアーぜ活性
Outline of Final Research Achievements

Fourteen CYPs which are drug metabolizing enzymes in human liver were reacted to more than eight kinds of adrenal steroids and their metabolites were identified. As a result, only CYP3A4 showed a various metabolite on HPLC chart. Production of androstendion from 17alpha-hydroxyprogesterone and 11-deoxycortisol by CYP3A4 was confirmed by GC-MS. It was revealed that CYP3A4 can catalyze side chain cleavage reaction (Lyase activity) to the steroids. 6beta-Hydoxy metabolite which were main metabolite from 11-deoxycortisol was identified. Then, Km and Vmax of 6beta-hydroxylase activity and Lyase activity against 11-deoxycortisol were determined and compared.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (1 results)

All 2015

All Presentation (1 results)

  • [Presentation] ヒトCYP3A4の新たなステロイド代謝活性とその意義2015

    • Author(s)
      片桐 昌直, 今井 健太, 渡辺 綾乃, 本間 桂子
    • Organizer
      第38回日本分子生物学会年会/第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2015-12-04
    • Related Report
      2015 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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