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Direct label-free measurement of the distribution of small molecular weight compound inside thick biological tissue using coherent Raman microspectroscopy

Research Project

Project/Area Number 25670089
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General anatomy (including histology/embryology)
Research InstitutionTokyo Medical and Dental University

Principal Investigator

Kawagishi Masahiko  東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (60323606)

Co-Investigator(Kenkyū-buntansha) TERADA Sumio  東京医科歯科大学, 大学院医歯学総合研究科, 教授 (00262022)
齊藤 健太  東京医科歯科大学, 大学院医歯学総合研究科, 助教 (60374659)
Co-Investigator(Renkei-kenkyūsha) MISAWA Kazuhiko  東京農工大学, 工学研究院, 教授 (80251396)
SUZUKI Takayuki  明治大学, 理工学部, 准教授 (80539510)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords非線型ラマン分光 / CARS / 顕微鏡技術 / 解剖学
Outline of Final Research Achievements

Distributions of small molecular weight (less than 300 kDa) compounds inside biological tissue have been obscure because of the lack of appropriate methods to measure them. We used CARS (Coherent Anti-Stokes Raman Scattering) spectroscopy to detect and identify a label-free small molecule compound. To facilitate detection in aqueous environment, we utilised time-resolved and phase-sensitive techniques to reduce non-resonant background generated from water. We applied this technique to detect small molecular weight compound, taurine, inside mouse cornea tissue immersed in taurine solution as an initial model experiment. We detected a Raman peak of taurine near wavenumber 1033 cm-1 inside cornea and successfully characterised its depth profile in the tissue. Our CARS spectra measurement can be a promising method to measure and visualise the distribution of small bio-related compounds in biological background without using any labeling.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (7 results)

All 2015 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) (of which Int'l Joint Research: 2 results) Remarks (2 results)

  • [Journal Article] Direct label-free measurement of the distribution of small molecular weight compound inside thick biological tissue using coherent Raman microspectroscopy2015

    • Author(s)
      Masahiko Kawagishi, Yuki Obara, Takayuki Suzuki, Masumi Hayashi, Kazuhiko Misawa & Sumio Terada
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 13868-13868

    • DOI

      10.1038/srep13868

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Direct Label-Free Measurement of the Distribution of Small Molecular Weight Compound Inside Thick Biological Tissue Using Coherent Raman Microspectroscopy2015

    • Author(s)
      Masahiko Kawagishi, Yuki Obara, Takayuki Suzuki, Masumi Hayashi, Kazuhiko Misawa and Sumio Terada
    • Organizer
      The 2nd East-Asia Microscopy Conference
    • Place of Presentation
      姫路商工会議所 (兵庫県・姫路市)
    • Year and Date
      2015-11-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Measuring the distribution of small molecule compounds inside biological tissue via intrinsic molecular vibrations using nonlinear Raman spectroscopy2015

    • Author(s)
      Masahiko Kawagishi, Yuki Obara, Takayuki Suzuki, Masumi Hayashi, Kazuhiko Misawa, Sumio Terada
    • Organizer
      第38回 日本神経科学大会
    • Place of Presentation
      神戸国際会議場, 神戸国際展示場 (兵庫県・神戸市)
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Measuring the distribution of small molecule compounds inside biological tis sue via intrinsic molecular vibrations using nonlinear Raman spectroscopy2015

    • Author(s)
      Kawagishi, Masahiko; Obara, Yuki; Suzuki, Takayuki; Hayashi, Masumi; Misawa, Kazuhiko; Terada, Sumio
    • Organizer
      第120回日本解剖学会総会・全国学術集会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report
  • [Presentation] MEASURING THE DISTRIBUTION OF TAURINE MOLECULE INSIDE BIOLOGICAL TISSUE VIA INTRINSIC MOLECULAR VIBRATIONS USING NONLINEAR RAMAN SPECTROSCOPY2015

    • Author(s)
      Masahiko Kawagishi, Yuki Obara, Takayuki Suzuki, Masumi Hayashi, Kazuhiko Misawa, Sumio Terada.
    • Organizer
      Biophysical Society 59th Annual Meeting
    • Place of Presentation
      Baltimore, Maryland, USA
    • Year and Date
      2015-02-07 – 2015-02-11
    • Related Report
      2014 Research-status Report
  • [Remarks] 「マウス組織に投与した低分子化合物の濃度分布測定に成功」【寺田純雄 教授】

    • URL

      http://www.tmd.ac.jp/archive-tmdu/kouhou/20150911.pdf

    • Related Report
      2015 Annual Research Report
  • [Remarks] 〔2015年9月8日リリース〕マウス組織に投与した低分子化合物の濃度分布測定に成功

    • URL

      http://www.tuat.ac.jp/disclosure/pressrelease/20150409130023/20150911/index.html

    • Related Report
      2015 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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