| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
We constructed gene expression vector systems, which allow transient expression of slug/sox9 genes known to force mammary epithelial cells to dedifferentiate into mammary stem cells. We observed, as reported, that slug/sox9 expressed MEC reconstituted proper mammary ducts when transplanted, although the efficiency was quite low. To solve this problem, we changed donor mice to those expressing rtTA, established culture technology for maintaining mammary stem cells (MaSC), and screened genes enhancing efficiency of dedifferentiation. Among them, we successfully established the MaSC culture technology. In addition, we newly produced a reporter mouse for stemness. With these technologies, we now established screening system of genes enhancing dedifferentiation, easily, rapidly, and efficiently. However, we have not yet discovered any new direct reprogramming genes strongly leading to produce MaSCs.
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