Artificial induction of the property of FRC in lymph node paracortex
| Project/Area Number |
25670101
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Niigata University (2014-2015)
Kansai Medical University (2013) |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | CCL21 / ケモカイン / ストローマ細胞 / 発現制御 / リンパ節 |
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| Outline of Final Research Achievements |
The paracortical region of lymph node (LN) is a prime site where antigen-specific interactions between T cells and dendritic cells takes place, therefore it plays a crucial role in the induction of adoptive immunity. Tissue structure of the paracortex is supported by fibroblastic reticular cells (FRCs) that produce CCL21, an important chemokine for attracting T cells and dendritic cells. However, mechanism for the selective expression of CCL21 in FRCs remains to be elucidated. The purpose of this study is to clarifying the molecular process. To identify the regulatory factors critical for the expression of CCL21, candidate genes highly expressing in mouse LN stromal cells were stably transfected to FRC cell lines established from LN using retroviral vectors. Although some transfectants showed alterations in responsibility to inflammatory cytokines, we unfortunately could not found the settings that induce CCL21 expression in FRC cell lines.
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Report
(4 results)
Research Products
(18 results)
