| Project/Area Number |
25670102
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Kurume University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OHTA Keisuke 久留米大学, 医学部, 准教授 (00258401)
KANAZAWA Tomonoshin 久留米大学, 医学部, 講師 (50529518)
RIKIMARU Yukiko 久留米大学, 医学部, 助教 (90368960)
HIGASHI Ryuhei 久留米大学, 医学部, 技能職員 (70569516)
TOGO Akinobu 久留米大学, 医学部, 技能職員 (80569517)
HAYASHI Tokumasa 久留米大学, 医学部, 講師 (20341357)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 線維芽細胞 / 間葉組織 / 平滑筋 / 次世代電子顕微鏡 / FIB/SEM / ブロック表面SEM観察 / 連続精密切削画像 / 三次元超微形態再構築 / ブロック表面SEM観察(BFI) |
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| Outline of Final Research Achievements |
We analyzed the three-dimensional (3D) ultrastructure of intestinal stromal cells by FIB/SEM, a novel scanning electron microscope technique, which enabled to establish 3D ultrastructure of the cells; shape, size, distribution pattern, and spatial relationship between neighboring cells and surrounding tissues. Our observations revealed that the stromal cells which had previously been considered as bipolar or stellate in shape by their long thin cytoplasmic processes in each single section, were in fact cells with broad, thin and wavy “sheet-like” cytoplasmic processes. They approached one another and appose each other at several points along the edge to form an incomplete wall within the connective tissue. Thus these stromal cell structures seem to create spatial divisions to form functional units within the tissue (compartmentalization). Similar structures known in other connective tissues are the future target to understand the cellular architecture of the connective tissue.
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