| Project/Area Number |
25670107
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | University of Fukui |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HIRAI Yoshikazu 京都大学, 大学院工学系研究科, 助教 (40452271)
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| Co-Investigator(Renkei-kenkyūsha) |
TABATA Osamu 京都大学, 大学院工学系研究科, 教授 (20288624)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | Single molecule / Ion channel protein / dynamics / 1分子計測 / イオンチャネル / 蛋白質 / MEMS |
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| Outline of Final Research Achievements |
In this project we developed the Diffracted Xray Tracking(DXT) method,which can measure single-molecule conformational changes of ion channel proteins, by applying the photolithography technique to a probe and a recording chamber. In the DXT method a gold nanocrystal was attached to an ion channel protein as a probe, and the diffraction spots from the nanocrystal was elicited by radiation of the synchrotron white xray. Tracking the motions on the detector plane can be translated into the protein motions. We found the methods for fabrications of the size-controlled probe in nano-meter scale and the recording chamber with low xray-scattering noise in micrometer scale by using photolithography technique.
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