| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
To understand the mechanism of thermosensation in our body, we analyzed structure-function of TRP channels that have a major role in thermosensation in the peripheral sensory nervous system. We analyzed electrophysiological properties of wild-type TRP channels and the mutants expressed in oocytes. We expressed and purified coiled-coil domain proteins of TRPV1 and TRPM8 and the ankyrin domain protein of TRPA1, that are reported to be involved in temperature sensing, with E. coli expression system. We also succeeded in determining the high resolution crystal structures of the coiled-coil domains of TRPM2 and TRPM3. We analyzed thermal stabilities of the coiled-coil domain proteins.
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