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Stabilization of KvLQT1 by Hsp70 in differentiiated cardiomyocytes derived from LQT1 iPS cells

Research Project

Project/Area Number 25670110
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General physiology
Research InstitutionTottori University

Principal Investigator

HISATOME Ichiro  鳥取大学, 医学(系)研究科(研究院), 教授 (60211504)

Co-Investigator(Kenkyū-buntansha) SHIRAYOSHI Yasuaki  鳥取大学, 医学研究科, 准教授 (90249946)
IKEDA Nobuhito  鳥取大学, 医学研究科, 助教 (50620316)
MIAKE Junichiro  鳥取大学, 医学部, 講師 (40372677)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords疾患iiPS / LQT1 / 熱ショック蛋白 / iPS cell / LQT / ion channel / 疾患iPS細胞 / HSP70
Outline of Final Research Achievements

研究成果の概要(英文):We identified the novel missense mutation M437V of KCNQ1 in a patient with QT interval prolongation (Long QT type 1: LQT1).We employed iPS cell (iPSC)-derived cardiomyocytes to investigate the electrophysiological properties of the mutant channel in LQT1 cardiomyocytes and to see effects of heat shock proteins. To generate iPSCs from the patient and a normal subject, peripheral blood T cells were reprogrammed. Differentiated cardiomyocytes from LQT1 iPSCs exhibited prolongation of action potential duration (APD), which was due to a reduction of the KCNQ1-mediated current IKs; Na+ and Ca2+ channel currents, as well as K+ channel currents other than IKs were comparable to those of cardiomyocytes from normal iPSCs and human ESCs. We tried to examine the heat shock, heat shock factor1 as well as heat shock protein 70 on the mutant channel properties of cardiomyocytes from LQT1 iPS cells.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (12 results)

All 2014 2013 2012

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (6 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Instability of KCNE1-D85N that Causes Long QT Syndrome: Stabilization by Verapamil2014

    • Author(s)
      Sakata S, Kurata Y, Li P, Notsu T, Morikawa K, Miake J, Higaki K, Yamamoto Y, Yoshida A, Shirayoshi Y, Yamamoto K, Horie M, Ninomiya H, Kanzaki S, Hisatome I.
    • Journal Title

      Pacing Clinical Electrophysiology

      Volume: Epub ahead of print Issue: 7 Pages: 1-11

    • DOI

      10.1111/pace.12360

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Heterochromatin protein 1γ overexpression in P19 embryonal carcinoma cells elicits spontaneous differentiation into the three germ layers.2013

    • Author(s)
      Morikawa K, Ikeda N, Hisatome I, Shirayoshi Y.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 431(2) Issue: 2 Pages: 225-31

    • DOI

      10.1016/j.bbrc.2012.12.128

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hsp90 prevents interaction between CHIP and HERG proteins to facilitate maturation of wild-type and mutant HERG proteins2013

    • Author(s)
      Iwai C, P Li,
    • Journal Title

      Cardiovasc Res

      Volume: 3 Issue: 3 Pages: 520-528

    • DOI

      10.1093/cvr/cvt200

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Simultaneous Treatment with Azelnidipine and Olmesartan Inhibits Apoptosis of Hl-1 Cardiac Myocytes Expressing E334k cMyBPC2013

    • Author(s)
      Bahrudin U, Ikeda N, Utami SB, Maharani N, Morikawa K, Li P
    • Journal Title

      Drug Res (Stuttg).

      Volume: 10 Issue: 10 Pages: 515-520

    • DOI

      10.1055/s-0033-1347188

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of hyperpolarization-activated current If on robustness of sinoatrial node pacemaking: theoretical study on influence of intracellular Na+ concentration2012

    • Author(s)
      Kurata Y
    • Journal Title

      Am J Physiol Heart Circ Physiol

      Volume: 304 Issue: 10 Pages: 61-7

    • DOI

      10.1152/ajpheart.00777.2012

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Stabilization of A78T-HERG that Causes Long QT Syndrome Type2 by Heat Shock Protein Family2014

    • Author(s)
      TakehitoKondo, JunichiroMiake, Kazuyoshi Ogura, Masaru Kato, Kazuhiko Iitsuka, Kazuhiro Yamamoto Yasuaki Shirayoshi, Ichiro Hisatome.
    • Organizer
      第29回日本不整脈学会・第31回日本心電学会学術集会
    • Place of Presentation
      東京フォーラム 東京都千代田区
    • Year and Date
      2014-07-22 – 2014-07-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] Electrical properties of human iPS cell-derived cardiac myocytes from the patient with long QT syndrome harboring a novel mutation of KCNQ1.2014

    • Author(s)
      十川達文、一ノ瀬貴史、森川久未、池田信人、白吉安昭、倉田康孝、三明淳一朗、山本一博、湯浅慎介、福田恵一、久留一郎
    • Organizer
      第29回日本不整脈学会・第31回日本心電学会学術集会
    • Place of Presentation
      東京フォーラム 東京都千代田区
    • Year and Date
      2014-07-22 – 2014-07-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] Hsp90 Prevents Interaction between CHIP and HERG Proteins to Facilitate Maturation of Wild-type and Mutant HERG Proteins2014

    • Author(s)
      Peili Li, Yasutaka Kurata, Nani Maharani, Yuko Ishido, Juichiro Miake, Yasuaki Shirayoshi, Kazuhiro Yamamoto, Ichiro Hisatome
    • Organizer
      第29回日本不整脈学会・第31回日本心電学会学術集会
    • Place of Presentation
      東京フォーラム 東京都千代田区
    • Year and Date
      2014-07-22 – 2014-07-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] QT延長症候群における早期後脱分極発現の力学的機序:ヒト心室筋細胞モデルの分岐構造解析による検証2013

    • Author(s)
      倉田 康孝.久留 一郎.谷田 守.芝本 利重
    • Organizer
      第30回日本心電学会学術集会
    • Place of Presentation
      横浜市 パシフィコ横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] E3 ligase CHIP interacts with Hsc70 to regulate Kv1.5 expression and repolarization in HL-1 cells2013

    • Author(s)
      Peili Li, Masaru Kato, Junichiro Miake, Kazuhiro Yamamoto, Yasuaki Shirayoshi, Ichiro Hisatome
    • Organizer
      第30回日本心電学会学術集会
    • Place of Presentation
      横浜市 パシフィコ横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒト末梢血T細胞由来人工多能性幹(hTiPS)細胞の樹立と心筋分化誘導ならびにその特性解析2013

    • Author(s)
      十川 達文.三明 淳一朗.山本 ―博.白吉 安昭.久留 一郎
    • Organizer
      第103回日本循環器学会中国地方会
    • Place of Presentation
      米子市 米子ビッグシップ
    • Related Report
      2013 Research-status Report
  • [Patent(Industrial Property Rights)] 新規ペースメーカ細胞2014

    • Inventor(s)
      池田信人、久留一郎
    • Industrial Property Rights Holder
      池田信人、久留一郎
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-198466
    • Filing Date
      2014-12-25
    • Related Report
      2014 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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