Continuous pH measurement of interstitial fluid and clarification of pathogenic mechanism of insulin resistance by ISFET pH sensor
| Project/Area Number |
25670111
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
MARUNAKA Yoshinori 京都府立医科大学, 医学(系)研究科(研究院), 教授 (00127036)
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| Co-Investigator(Kenkyū-buntansha) |
NIISATO Naomi 京都府立医科大学, 医学研究科, 准教授 (00237645)
MIYAZAKI Hiroaki 京都府立医科大学, 医学研究科, 講師 (30360027)
HOSOGI Shigekuni 京都府立医科大学, 医学研究科, 助教 (30405261)
細木 誠之 京都府立医科大学, 医学(系)研究科(研究院), その他 (30433254)
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| Co-Investigator(Renkei-kenkyūsha) |
HIDESHIMA Sho 早稲田大学, 大学院理工学術院, 助教 (10580433)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | pH / 糖尿病 / 組織間質液 / インスリン抵抗性 |
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| Outline of Final Research Achievements |
A) Developments of collection methods of interstitial fluid and methods approaching to interstitial fluid. We succeeded in two-day insertion of syringe with outer diameter of 0.80 mm and inner diameter of 0.57 mm in interstitial fluid space.
B) pH measurements of interstitial fluid. Even if we tried to obtain the interstitial fluid using the syringe, we could not get the interstitial fluid due to clogged inner spaces of the syringe.Therefore, we tried to directly measure pH of interstitial fluids using micro pH meters,ISFET pH sensor and antimony pH sensor.We could not obtain any stable pH value of interstitial fluid using ISFET pH sensor, however stable values of interstitial fluid pH were obtained by antimony pH sensor.Using antimony pH sensor, we obtained pH of 7.21 ± 0.03 in diabetic rats and pH of 7.36 ± 0.02 in non-diabetic rats（n = 6; p < 0.05）.
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Report
(3 results)
Research Products
(50 results)
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[Journal Article] PPARα autocrine regulation of Ca²⁺-regulated exocytosis in guinea pig antral mucous cells: NO and cGMP accumulation2014
Author(s)
Tanaka S, Sugiyama N, Takahashi Y, Mantoku D, Sawabe Y, Kuwabara H, Nakano T, Shimamoto C, Matsumura H, MARUNAKA Y, Nakahari T
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Journal Title
Am J Physiol Gastrointest Liver Physiol
Volume: 307
Issue: 12
Pages: G1169-G1179
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Role of the Na+/K+/2Cl- cotransporter NKCC1 in cell cycle progression in human esophageal squamous cell carcinoma2014
Author(s)
Shiozaki A, Nako Y, Ichikawa D, Konishi H, Komatsu S, Kubota T, Fujiwara H, Okamoto K, Kishimoto M, MARUNAKA Y, Otsuji E.
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Journal Title
World J Gastroenterol (in press)
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] A PKG inhibitor increases Ca2+-regulated exocytosis in guinea pig antral mucous cells: cAMP accumulation via PDE2A inhibition2013
Author(s)
Tanaka S, Tanaka R, Harada S, Kohda Y, Matsumura H, Shimamoto C, Sawabe Y, MARUNAKA Y, Kuwabara H, Takahashi Y, Ito S, Nakahari T
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Journal Title
Am J Physiol Gastrointest Liver Physiol
Volume: 304
Issue: 9
Pages: G773-G780
DOI
Related Report
Peer Reviewed
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[Presentation] What is the role of interstitial pH in diabetes mellitus? Improving action of propolis on DM symptom via pH regulation2013
Author(s)
MARUNAKA Y, Aoi W, Hosogi S, Niisato N, Yokoyama N, Hayata H, Miyazaki H, Kusuzaki K, Taruno A, Nomura T.
Organizer
The 16th International Symposium on Molecular Medicine
Place of Presentation
Creta Maris, Hersonissos, Crete, Greece
Related Report
Invited
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