| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
G protein-coupled receptor kinases (GRKs) have been known as kinases that phosphorylate G protein-coupled receptors (GPCRs) and lead to desensitization of the receptors. We found that the expression levels of several genes involved in the molecular mechanisms of circadian clock are altered in the hearts of GRK5 KO mice. To investigate the contribution of GRK5 to the circadian clock machinery, we examined whether GRK5 bind to circadian clock proteins. By immunoprecipitation assay, we found that GRK5 interacts with clock proteins, PER1/2/3 and CRY1/2. We also found that mRNA level of GRK5 displays circadian rhythm in the suprachiasmatic nucleus that is located in the hypothalamus and is responsible for controlling circadian rhythms of our whole body.
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