Establishment of gene expression system in Bombyx mori

• Project/Area Number: 25670134
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Hokkaido University
• Principal Investigator: HATAKEYAMA Shigetsugu 北海道大学, 医学(系)研究科(研究院), 教授 (70294973)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ゲノム医科学 / 遺伝学 / 遺伝子 / 昆虫 / タンパク質 / ウイルス

Outline of Final Research Achievements

It is important to use animal models in order to analyze the etiology of human diseases in medical researches. We tried to establish gene expression system in Bombyx more to analyze genetic diseases. We established viral expression system using special Bombyx strains, which can be infected by AcNPV. We made AcNPV virus containing a target gene (GFP gene as a model gene), infected the virus and then could express GFP protein in pupae of Bombyx.

Research Products

• [Journal Article] Molecular role of RNF43 in canonical and noncanonical Wnt signaling (2015)
  • Author(s): Tsukiyama, T., Fukui, A., Terai, S., Fujioka, Y., Shinada, K., Takahashi, H., Yamaguchi, T.P., Ohba, Y., Hatakeyama, S.
  • Journal Title: Mol. Cell. Biol. Volume: 未定
  • NAID: 120005672365
  • Peer Reviewed
• [Journal Article] The inflammatory cytokine IL-1b is involved in bladder remodeling after bladder outlet obstruction in mice (2015)
  • Author(s): Kanno, Y., Mitsui, T., Kitta, T., Moriya, K., Tsukiyama, T., Hatakeyama, S. and Nonomura, K.
  • Journal Title: Neurouro. Urodyn. Volume: 未定 Issue: 3 Pages: 277-381
  • DOI: 10.1002/nau.22721
  • Peer Reviewed
• [Journal Article] MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex (2015)
  • Author(s): Takahashi, H., Takigawa, I., Watanabe, M., Anwar, D., Shibata, M., Tomomori-Sato, C., Sato, S., Ranjan, A., Seidel, C.W., Tsukiyama, T., Mizushima, W., Hayashi, M., Ohkawa, Y., Conaway, J.W., Conaway, R.C. and Hatakeyama, S
  • Journal Title: Nature Commun. Volume: 5 Issue: 1 Pages: 5941-5941
  • DOI: 10.1038/ncomms6941
  • NAID: 120005621881
  • Peer Reviewed / Open Access
• [Journal Article] The TRIM-FLMN protein TRIM45 directly interacts with RACK1 and negatively regulates PKC-mediated signaling pathway (2015)
  • Author(s): Sato, T., Takahashi, H., Hatakeyama, S., Iguchi, A. and Ariga, T
  • Journal Title: Oncogene Volume: 34 Issue: 10 Pages: 1280-1291
  • DOI: 10.1038/onc.2014.68
  • Peer Reviewed
• [Journal Article] Oxidative stress regulates IL-4 gene expression in mast cells through the reduction of histone deacetylase (2015)
  • Author(s): Nakamaru, Y., Takagi, D., Homma, A., Hatakeyama, S., Fukuda, S.
  • Journal Title: Otolaryngol. Head Neck Surg. Volume: 152 Issue: 1 Pages: 48-52
  • DOI: 10.1177/0194599814559189
  • Peer Reviewed / Open Access
• [Journal Article] Siglec-15 is a potential therapeutic target for postmenopausal osteoporosis (2015)
  • Author(s): Kameda, Y., Takahata, M., Mikuni, S., Shimizu, T., Hamano, H., Angata, T., Hatakeyama, S., Kinjo, M. and Iwasaki, N.
  • Journal Title: Bone Volume: 71 Pages: 217-226
  • DOI: 10.1016/j.bone.2014.10.027
  • Peer Reviewed
• [Journal Article] Pathology of frontotemporal dementia with limb girdle muscular dystrophy caused by DNAJB6 mutation (2014)
  • Author(s): Yabe, I., Tanino, M., Yaguchi, H., Takiyama, A., Cai, H., Kanno, H., Takahashi, I., Hayashi, Y., Watanabe, M., Takahashi, H., Hatakeyama, S., Tanaka, S. and Sasaki, H.
  • Journal Title: Clin. Neurol. Neurosur. Volume: 127 Pages: 10-12
  • DOI: 10.1016/j.clineuro.2014.09.013
  • NAID: 120005540291
  • Peer Reviewed / Open Access
• [Journal Article] TRIM29 suppresses TWIST1 and invasive breast cancer behavior (2014)
  • Author(s): Ai, L., Wan-Ju Kim, W., Alpay, M., Tang, M., Pardo, C.E., Hatakeyama, S., May, W.S. , Kladde, M.P., Heldermon, C.D., Siegel, E.M. and Brown, K.D.
  • Journal Title: Cancer Res Volume: 74 Issue: 17 Pages: 4875-4887
  • DOI: 10.1158/0008-5472.can-13-3579
  • Peer Reviewed
• [Journal Article] TRIM29 as a novel prostate basal cell marker for diagnosis of prostate cancer (2014)
  • Author(s): Kanno, Y., Watanabe, M., Kimura, T., Nonomura, K., Tanaka, S. and Hatakeyama, S
  • Journal Title: Acta Histochem Volume: 116 Issue: 5 Pages: 708-712
  • DOI: 10.1016/j.acthis.2013.12.009
  • NAID: 120005472205
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 14-3-3 proteins sequester a pool of soluble TRIM32 ubiquitin ligase to repress autoubiquitination and cytoplasmic body formation (2013)
  • Author(s): Ichimura T, Taoka M, Shoji I, Kato H, Sato T, Hatakeyama S, Isobe T and Hachiya N
  • Journal Title: J. Cell. Sci. Volume: 126 Pages: 2014-2026
  • DOI: 10.1242/jcs.122069
  • Peer Reviewed
• [Journal Article] Siglec-15 Regulates Osteoclast Cytoskeletal Organization in Association with Signaling Adaptor DAP12 (2013)
  • Author(s): Kameda Y, Takahata M, Komatsu M, Mikuni S, Hatakeyama S, Shimizu T, Angata T, Kinjo M, Minami A and Iwasaki N
  • Journal Title: J. Bone Mineral Res. Volume: 28 Issue: 12 Pages: 2463-2475
  • DOI: 10.1002/jbmr.1989
  • Peer Reviewed
• [Journal Article] Identification of anti-Sez6l2 antibody in a patient with cerebellar ataxia and retinopathy (2013)
  • Author(s): Yaguchi H, Yabe I, Takahashi H, Okumura F, Takeuchi A, Horiuchi, K, Kan T, Kanda, A, Saito W, Matsumoto M, Nakayama KI, Hatakeyama S and Sasaki H
  • Journal Title: J. Neurol. Volume: 261 Issue: 1 Pages: 224-226
  • DOI: 10.1007/s00415-013-7134-5
  • Peer Reviewed
• [Presentation] メディエーター複合体による転写伸長制御 (2014)
  • Author(s): 高橋秀尚, 瀧川一学, 渡部昌, Delnur Anwar, 柴田美音, 佐藤チエリ, 佐藤滋生, Amol Ranjan, Chris W Seidel, 築山忠維, 林正康、大川恭行, Joan W Conaway, Ronald C Conaway, 畠山鎮次
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Med26はLittle elongation complexをリクルートすることでsmall nuclear RNA遺伝子の発現を制御する (2014)
  • Author(s): 高橋秀尚, 瀧川一学, 渡部昌, Anwar Delnur, 柴田美音, 佐藤チエリ, 佐藤滋生, Ranjan Amol, Seidel Chris, 築山忠維, 林正康, 大川恭行, Conaway Joan, Conaway Ronald, 畠山鎮次
  • Organizer: 第87回日本生化学会
  • Place of Presentation: 京都国際会議場（京都府・京都市）
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] TRIMタンパク質による脂肪細胞分化制御 (2014)
  • Author(s): 渡部昌, 髙橋秀尚, 畠山鎮次
  • Organizer: 第87回日本生化学会
  • Place of Presentation: 京都国際会議場（京都府・京都市）
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] TRIM29はヒストンH2AXのリン酸化を介してDNA二本鎖切断修復を制御する (2014)
  • Author(s): 桝田安志, 髙橋秀尚, Conaway Ronald C., Conaway Joan W., 畠山鎮次
  • Organizer: 第87回日本生化学会
  • Place of Presentation: 京都国際会議場（京都府・京都市）
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] Tripartite motif protein39(TRIM39)の機能解析 (2014)
  • Author(s): 鈴木正宣，渡部昌，中丸裕爾，福田諭，畠山鎮次
  • Organizer: 第79回日本インターフェロン・サイトカイン学会学術集会
  • Place of Presentation: 北海道大学（北海道・札幌市）
  • Year and Date: 2014-06-19 – 2014-06-20
• [Presentation] Regulation of cellular functions by TRIM proteins (2013)
  • Author(s): Hatakeyama, S.
  • Organizer: The 35th Naito Conference: The Ubiquitin-Protesome System: from Basic Mechanisms to Pathophysiological Roles
  • Place of Presentation: シャトレーゼ ガトーキングダム札幌（札幌）
  • Invited
• [Presentation] Involvement of linear polyubiquitylation in PPARγ transcriptional activity (2013)
  • Author(s): Watanabe, M., Takayashi, H., Hatakeyama, S.
  • Organizer: The 35th Naito Conference: The Ubiquitin-Protesome System: from Basic Mechanisms to Pathophysiological Roles
  • Place of Presentation: シャトレーゼ ガトーキングダム札幌（札幌）
• [Presentation] Functional analysis of a novel multiprotein complex containing ataxia telangiectasia group D-complementing (ATDC) gene product, TRIM29 (2013)
  • Author(s): Masuda, Y., Takahashi, H., Matsumoto, M., Nakayama, K., Hatakeyama, S.
  • Organizer: The 35th Naito Conference: The Ubiquitin-Protesome System: from Basic Mechanisms to Pathophysiological Roles
  • Place of Presentation: シャトレーゼ ガトーキングダム札幌（札幌）
• [Presentation] TRIMタンパク質の多彩な機能 (2013)
  • Author(s): 畠山鎮次
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜（横浜）
  • Invited
• [Presentation] ATDCはヒストンH2A.Xのリン酸化とヒストンH4のアセチル化を介してDNA日本鎖切断修復を制御する (2013)
  • Author(s): 桝田安志、畠山鎮次
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜（横浜）
• [Presentation] がん抑制遺伝子RNF43は異なるメカニズムによってWnt/beta-catenin経路とnoncanonical Wnt経路を抑制する (2013)
  • Author(s): 築山忠維、畠山鎮次
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜）
• [Presentation] RNF43遺伝子変異によるがん抑制遺伝子からがん遺伝子への機能転換 (2013)
  • Author(s): 築山 忠維，福井 彰雅，寺井 小百合，藤岡 容一朗，品田 恵佐，Yamaguchi Terry，大場 雄介，畠山 鎮次
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（神戸）