Establishment of gene expression system in Bombyx mori
Project/Area Number |
25670134
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
General medical chemistry
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Research Institution | Hokkaido University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | ゲノム医科学 / 遺伝学 / 遺伝子 / 昆虫 / タンパク質 / ウイルス |
Outline of Final Research Achievements |
It is important to use animal models in order to analyze the etiology of human diseases in medical researches. We tried to establish gene expression system in Bombyx more to analyze genetic diseases. We established viral expression system using special Bombyx strains, which can be infected by AcNPV. We made AcNPV virus containing a target gene (GFP gene as a model gene), infected the virus and then could express GFP protein in pupae of Bombyx.
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Report
(3 results)
Research Products
(24 results)
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[Journal Article] Molecular role of RNF43 in canonical and noncanonical Wnt signaling2015
Author(s)
Tsukiyama, T., Fukui, A., Terai, S., Fujioka, Y., Shinada, K., Takahashi, H., Yamaguchi, T.P., Ohba, Y., Hatakeyama, S.
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Journal Title
Mol. Cell. Biol.
Volume: 未定
NAID
Related Report
Peer Reviewed
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[Journal Article] The inflammatory cytokine IL-1b is involved in bladder remodeling after bladder outlet obstruction in mice2015
Author(s)
Kanno, Y., Mitsui, T., Kitta, T., Moriya, K., Tsukiyama, T., Hatakeyama, S. and Nonomura, K.
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Journal Title
Neurouro. Urodyn.
Volume: 未定
Issue: 3
Pages: 277-381
DOI
Related Report
Peer Reviewed
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[Journal Article] MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex2015
Author(s)
Takahashi, H., Takigawa, I., Watanabe, M., Anwar, D., Shibata, M., Tomomori-Sato, C., Sato, S., Ranjan, A., Seidel, C.W., Tsukiyama, T., Mizushima, W., Hayashi, M., Ohkawa, Y., Conaway, J.W., Conaway, R.C. and Hatakeyama, S
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Journal Title
Nature Commun.
Volume: 5
Issue: 1
Pages: 5941-5941
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Siglec-15 is a potential therapeutic target for postmenopausal osteoporosis2015
Author(s)
Kameda, Y., Takahata, M., Mikuni, S., Shimizu, T., Hamano, H., Angata, T., Hatakeyama, S., Kinjo, M. and Iwasaki, N.
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Journal Title
Bone
Volume: 71
Pages: 217-226
DOI
Related Report
Peer Reviewed
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[Journal Article] Pathology of frontotemporal dementia with limb girdle muscular dystrophy caused by DNAJB6 mutation2014
Author(s)
Yabe, I., Tanino, M., Yaguchi, H., Takiyama, A., Cai, H., Kanno, H., Takahashi, I., Hayashi, Y., Watanabe, M., Takahashi, H., Hatakeyama, S., Tanaka, S. and Sasaki, H.
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Journal Title
Clin. Neurol. Neurosur.
Volume: 127
Pages: 10-12
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] TRIM29 suppresses TWIST1 and invasive breast cancer behavior2014
Author(s)
Ai, L., Wan-Ju Kim, W., Alpay, M., Tang, M., Pardo, C.E., Hatakeyama, S., May, W.S. , Kladde, M.P., Heldermon, C.D., Siegel, E.M. and Brown, K.D.
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Journal Title
Cancer Res
Volume: 74
Issue: 17
Pages: 4875-4887
DOI
Related Report
Peer Reviewed
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[Journal Article] TRIM29 as a novel prostate basal cell marker for diagnosis of prostate cancer2014
Author(s)
Kanno, Y., Watanabe, M., Kimura, T., Nonomura, K., Tanaka, S. and Hatakeyama, S
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Journal Title
Acta Histochem
Volume: 116
Issue: 5
Pages: 708-712
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Identification of anti-Sez6l2 antibody in a patient with cerebellar ataxia and retinopathy2013
Author(s)
Yaguchi H, Yabe I, Takahashi H, Okumura F, Takeuchi A, Horiuchi, K, Kan T, Kanda, A, Saito W, Matsumoto M, Nakayama KI, Hatakeyama S and Sasaki H
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Journal Title
J. Neurol.
Volume: 261
Issue: 1
Pages: 224-226
DOI
Related Report
Peer Reviewed
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[Presentation] メディエーター複合体による転写伸長制御2014
Author(s)
高橋秀尚, 瀧川一学, 渡部昌, Delnur Anwar, 柴田美音, 佐藤チエリ, 佐藤滋生, Amol Ranjan, Chris W Seidel, 築山忠維, 林正康、大川恭行, Joan W Conaway, Ronald C Conaway, 畠山鎮次
Organizer
第37回日本分子生物学会年会
Place of Presentation
パシフィコ横浜(神奈川県・横浜市)
Year and Date
2014-11-25 – 2014-11-27
Related Report
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[Presentation] Med26はLittle elongation complexをリクルートすることでsmall nuclear RNA遺伝子の発現を制御する2014
Author(s)
高橋秀尚, 瀧川一学, 渡部昌, Anwar Delnur, 柴田美音, 佐藤チエリ, 佐藤滋生, Ranjan Amol, Seidel Chris, 築山忠維, 林正康, 大川恭行, Conaway Joan, Conaway Ronald, 畠山鎮次
Organizer
第87回日本生化学会
Place of Presentation
京都国際会議場(京都府・京都市)
Year and Date
2014-10-15 – 2014-10-18
Related Report
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[Presentation] Functional analysis of a novel multiprotein complex containing ataxia telangiectasia group D-complementing (ATDC) gene product, TRIM292013
Author(s)
Masuda, Y., Takahashi, H., Matsumoto, M., Nakayama, K., Hatakeyama, S.
Organizer
The 35th Naito Conference: The Ubiquitin-Protesome System: from Basic Mechanisms to Pathophysiological Roles
Place of Presentation
シャトレーゼ ガトーキングダム札幌(札幌)
Related Report
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