Construction and application of linear ubiquitin-specific molecular probes for anti-cancer drug screening
| Project/Area Number |
25670137
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 酵素 / タンパク質 / 細胞・ 組織 / バイオテクノロジー / 生体機能利用 / シグナル伝達 / 免疫学 |
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| Outline of Final Research Achievements |
NF-κB signal plays a critical role on immune and inflammatory responses induced by various stimuli such as bacterial and viral infections. We identified a novel enzyme (named LUBAC) that generates head-to-tail-linked linear polyubiquitin chain, and LUBAC is critical for NF-κB regulation. Furthermore, we identified a protein domain (A20 ZF7), which exhibits linear ubiquitin-specific binding. We here employed the domain to detect intracellular linear ubiquitin and screening for linear ubiquitin-binding chemicals.
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Report
(3 results)
Research Products
(25 results)
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[Journal Article] Structures of CYLD USP with Met1- or Lys63-linked diubiquitin reveal mechanisms for dual specificity2015
Author(s)
Sato, Y., Goto, E., Shibata, Y., Kubota, Y., Yamagata, A., Goto-Ito, S., Kubota, K., Inoue, J., Takekawa, M., Tokunaga, F. and Fukai, S.
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Journal Title
Nat. Struct. Mol. Biol.
Volume: 22
Issue: 3
Pages: 222-229
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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