Analysis of dynamic functions of membrane microdomains by linkage of glycosphingolipids on the cell membrane and transcriptional regulation
Project/Area Number |
25670141
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
General medical chemistry
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Research Institution | Chubu University (2015) Nagoya University (2013-2014) |
Principal Investigator |
FURUKWA Koichi 中部大学, 生命健康科学部, 教授 (80211530)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | ガングリオシド / 転写因子 / セクレターゼ / ミクロドメイン / 脂質ラフト / メラノーマ / スフィンゴ糖脂質 / 細胞膜 / ネオジェニン / EMARS / 細胞質ドメイン |
Outline of Final Research Achievements |
Regulatory mechanisms by which interaction between GD3 and its associating molecule, neogenin on the cell surface of melanoma cells were analyzed with focus on the intracytoplasmic domain of neogenin (Ne-ICD). When a protease inhibitor was added to the culture medium, increased levels of Ne-ICD was detected in GD3+ cells, while it was hard to detect when a gamma-secretase inhibitor was added, suggesting that Ne-ICD was generated by recruiting gamma-secretase to lipid rafts by GD3 expression. By ChIP-sequencing, target genes that are driver by Ne-ICD have been identified, and those genes have been up-regulated by the introduction of Ne-ICD cDNA.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Ganglioside GD3 Enhances Invasiveness of Gliomas by Forming a Complex with Platelet-derived Growth Factor Receptor α and Yes Kinase.2015
Author(s)
Ohkawa Y, Momota H, Kato A, Hashimoto N, Tsuda Y, Kotani N, Honke K, Suzumura A, Furukawa K, Ohmi Y, Natsume A, Wakabayashi T, Furukawa K.
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Journal Title
J Biol Chem.
Volume: 290
Issue: 26
Pages: 16043-16058
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Akt-girdin signaling in cancer-associated fibroblasts contributes to tumor progression2015
Author(s)
Yumiko Yamamura, Naoya Asai, Atsushi Enomoto, Takuya Kato, Shinji Mii, Yuji Kondo, Kaori Ushida, Kaoru Niimi, Nobuyuki Tsunoda, Masato Nagino, Shu Ichihara, Koichi Furukawa, Kengo Maeda, Toyoaki Murohara, Masahide Takahashi
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Journal Title
Cancer Research
Volume: 75
Issue: 5
Pages: 813-823
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Neuronal Expression of GalNAc Transferase Is Sufficient to Prevent the Age-Related Neurodegenerative Phenotype of Complex Ganglioside-Deficient Mice2014
Author(s)
Denggao Yao, Rhona McGonigal, Jennifer A. Barrie, Joanna Cappell, Madeleine E. Cunningham, Gavin R. Meehan, Simon N. Fewou, Julia M. Edgar, Edward Rowan, Yuhsuke Ohmi, Keiko Furukawa, Koichi Furukawa, Peter J. Brophy, Hugh J. Willison
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Journal Title
The journal of neuroscience
Volume: 34
Issue: 3
Pages: 880-891
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Neuronal expression of GalNAc transferase is sufficient to prevent the age-related neurodegenerative phenotype of complex ganglioside deficient mice.2014
Author(s)
Yao, D., McGonigal, R., Barrie, J.A., Cappell, J., Cunningham, M.E., Meehan, G.R., Fewou, S.N., Edgar, J.M., Rowan, E., Ohmi, Y., Furukawa, K., Furukawa, K., Brophy, P.J., Willison, H.J.
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Journal Title
J. Neurosci.
Volume: 34
Pages: 880-891
Related Report
Peer Reviewed
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[Journal Article] β4GalT6 is involved in the synthesis of lactosylceramide with less intensity than β4GalT52013
Author(s)
Tokuda N, Numata S, Li X, Nomura T, Takizawa M, Kondo Y, Yamashita Y, Hashimoto N, Kiyono T, Urano T, Furukawa K, Furukawa K
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Journal Title
Glycobiology
Volume: 23
Issue: 10
Pages: 1175-1183
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Book] Lipid Rafts2014
Author(s)
Furukawa K, Ohmi Y, Ohkawa Y, Kondo Y, Ohkawa Y, Hashimoto N, Tajima O, Furukawa K
Total Pages
268
Publisher
Nova Science Publishers
Related Report
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