| Project/Area Number |
25670145
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Ehime University |
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Principal Investigator |
AOTO Mamoru 愛媛大学, 医学(系)研究科(研究院), 准教授 (50372727)
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| Project Period (FY) |
2013-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 細胞内シグナル伝達 / 赤血球分化 |
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| Research Abstract |
I examined a condition to produce human BclxL to mouse erythroid progenitor cells as a preliminary experiment about the production of the mouse immortalized erythroid progenitor cells. When the human BclxL expression plasmid was introduced into mouse erythroid progenitor cells by electroporation, it was revealed that expression of the human BclxL protein was very low. Then, when the promoter of the expression plasmid was changed into the EF-1alpha promoter instead of the CMV promoter, the expression level of human BclxL protein increased significantly.
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