Analysis of the role of proliferation in cellular reprogramming
| Project/Area Number |
25670146
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
SUZUKI Atsushi 九州大学, 生体防御医学研究所, 教授 (30415195)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 細胞 / リプログラミング / 増殖 / 再生医療 |
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| Outline of Final Research Achievements |
To examine the relationship between direct reprogramming of fibroblasts into induced hepatocyte-like (iHep) cells and cellular proliferation, we sequentially analyzed the early stage of fibroblast conversion into iHep cells using live-cell imaging, immunofluorescence staining, and quantitative polymerase chain reaction analyses. Our data demonstrated that the conversion into iHep cells began within 48 hours after introduction of the transgenes into fibroblasts, and the number of iHep cells increased gradually as the culture progressed. The rapid cell-fate conversion of fibroblasts into iHep cells and stable expansion of iHep cells will be useful in the future cell transplantation therapy and screening of drugs for patients with liver diseases.
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Report
(3 results)
Research Products
(39 results)
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[Journal Article] Transcription factors interfering with dedifferentiation induce cell type-specific transcriptional profiles.2013
Author(s)
Hikichi T, Matoba R, Ikeda T, Watanabe A, Yamamoto T, Yoshitake S, Tamura-Nakano M, Kimura T, Kamon M, Shimura M, Kawakami K, Okuda A, Okochi H, Inoue T, Suzuki A, Masui S.
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Journal Title
Proceeding of the National Academy of Sciences of USA
Volume: 110
Issue: 16
Pages: 6412-6417
DOI
NAID
Related Report
Peer Reviewed
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