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Analysis of the role of proliferation in cellular reprogramming

Research Project

Project/Area Number 25670146
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General medical chemistry
Research InstitutionKyushu University

Principal Investigator

SUZUKI Atsushi  九州大学, 生体防御医学研究所, 教授 (30415195)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords細胞 / リプログラミング / 増殖 / 再生医療
Outline of Final Research Achievements

To examine the relationship between direct reprogramming of fibroblasts into induced hepatocyte-like (iHep) cells and cellular proliferation, we sequentially analyzed the early stage of fibroblast conversion into iHep cells using live-cell imaging, immunofluorescence staining, and quantitative polymerase chain reaction analyses. Our data demonstrated that the conversion into iHep cells began within 48 hours after introduction of the transgenes into fibroblasts, and the number of iHep cells increased gradually as the culture progressed. The rapid cell-fate conversion of fibroblasts into iHep cells and stable expansion of iHep cells will be useful in the future cell transplantation therapy and screening of drugs for patients with liver diseases.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (39 results)

All 2015 2014 2013 Other

All Journal Article (10 results) (of which Peer Reviewed: 9 results,  Acknowledgement Compliant: 5 results,  Open Access: 4 results) Presentation (27 results) (of which Invited: 15 results) Remarks (2 results)

  • [Journal Article] Liver regeneration: a unique and flexible reaction depending on the type of injury2015

    • Author(s)
      Suzuki A.
    • Journal Title

      Genes Cells

      Volume: 20 Issue: 2 Pages: 77-84

    • DOI

      10.1111/gtc.12200

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Dynamic three-dimensional morphogenesis of intrahepatic bile ducts in mouse liver development2015

    • Author(s)
      Takashima Y., Terada M., Kawabata M., Suzuki A.
    • Journal Title

      Hepatology

      Volume: 61 Issue: 3 Pages: 1003-1011

    • DOI

      10.1002/hep.27436

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Evidence of cell-fate conversion from hepatocytes to cholangiocytes in the injured liver: in vivo genetic lineage-tracing approaches2015

    • Author(s)
      Suzuki A.
    • Journal Title

      Curr Opin Gastroenterol

      Volume: 31 Issue: 3 Pages: 247-251

    • DOI

      10.1097/mog.0000000000000172

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hepatocytes, rather than cholangiocytes, can be the major source of primitive ductules in the chronically injured mouse liver2014

    • Author(s)
      Sekiya S. and Suzuki A.
    • Journal Title

      Am J Pathol

      Volume: 184 Issue: 5 Pages: 1468-1478

    • DOI

      10.1016/j.ajpath.2014.01.005

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Acquisition of lipid metabolic capability in hepatocyte-like cells directly induced from mouse fibroblasts2014

    • Author(s)
      Miura S. and Suzuki A.
    • Journal Title

      Front Cell Dev Biol

      Volume: 2 Pages: 1-6

    • DOI

      10.3389/fcell.2014.00043

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Direct reprogramming2014

    • Author(s)
      Suzuki A.
    • Journal Title

      Inflammation and Regeneration

      Volume: 34 Issue: 5 Pages: 209-210

    • DOI

      10.2492/inflammregen.34.209

    • NAID

      130004943872

    • ISSN
      1880-8190, 1880-9693
    • Related Report
      2014 Annual Research Report
    • Open Access
  • [Journal Article] Rapid cell-fate conversion of mouse fibroblasts into hepatocyte-like cells2014

    • Author(s)
      Miura S. and Suzuki A.
    • Journal Title

      Inflammation and Regeneration

      Volume: 34 Issue: 5 Pages: 211-216

    • DOI

      10.2492/inflammregen.34.211

    • NAID

      130004943873

    • ISSN
      1880-8190, 1880-9693
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Transcription factors interfering with dedifferentiation induce cell type-specific transcriptional profiles.2013

    • Author(s)
      Hikichi T, Matoba R, Ikeda T, Watanabe A, Yamamoto T, Yoshitake S, Tamura-Nakano M, Kimura T, Kamon M, Shimura M, Kawakami K, Okuda A, Okochi H, Inoue T, Suzuki A, Masui S.
    • Journal Title

      Proceeding of the National Academy of Sciences of USA

      Volume: 110 Issue: 16 Pages: 6412-6417

    • DOI

      10.1073/pnas.1220200110

    • NAID

      120005244176

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Regulation of organogenesis and stem cell properties by T-box transcription factors2013

    • Author(s)
      Takashima Y. and Suzuki A.
    • Journal Title

      Cell Mol Life Sci

      Volume: 70 Issue: 20 Pages: 3929-3945

    • DOI

      10.1007/s00018-013-1305-5

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Artificial induction and disease-related conversion of the hepatic fate2013

    • Author(s)
      Suzuki A.
    • Journal Title

      Curr Opin Genet Dev

      Volume: 23 Issue: 5 Pages: 579-584

    • DOI

      10.1016/j.gde.2013.04.006

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Direct reprogrammingによる肝細胞の直接誘導2015

    • Author(s)
      鈴木淳史
    • Organizer
      第14回日本再生医療学会総会シンポジウム「リプログラミングと多能性幹細胞」
    • Place of Presentation
      横浜
    • Year and Date
      2015-03-19 – 2015-03-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] iHep細胞研究から見出された肝細胞分化の新規制御機構2015

    • Author(s)
      三浦静、鈴木淳史
    • Organizer
      第14回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2015-03-19 – 2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] ダイレクトリプログラミングによって誘導された肝細胞様細胞の成熟化2015

    • Author(s)
      山本純平、鈴木淳史
    • Organizer
      第14回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2015-03-19 – 2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Direct reprogrammingによる肝細胞の直接誘導2014

    • Author(s)
      鈴木淳史
    • Organizer
      第2回細胞凝集研究会
    • Place of Presentation
      福岡
    • Year and Date
      2014-12-06
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 線維芽細胞から肝細胞へのダイレクトリプログラミング2014

    • Author(s)
      鈴木淳史
    • Organizer
      第87回日本生化学会大会「創薬や再生医療の基盤となる「動くクロマチン構造」を追う」
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Genetic cell lineage tracing in liver regeneration and cancer2014

    • Author(s)
      Suzuki, A.
    • Organizer
      2014 International Symposium of Materials on Regenerative Medicine (2014 ISOMRM)
    • Place of Presentation
      Tao-Yuan, Taiwan
    • Year and Date
      2014-08-27 – 2014-08-29
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 「ダイレクトリプログラミング」の現状と展望2014

    • Author(s)
      鈴木淳史
    • Organizer
      第35回日本炎症・再生医学会
    • Place of Presentation
      沖縄
    • Year and Date
      2014-07-01 – 2014-07-04
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 肝臓特異的Hhex遺伝子欠失マウス肝臓における嚢胞発生とWntシグナル2014

    • Author(s)
      塩尻信義、上野友也、福地智一、鈴木淳史、山本太一、野口民夫、小池亨
    • Organizer
      第21回肝細胞研究会
    • Place of Presentation
      東京
    • Year and Date
      2014-06-27 – 2014-06-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] iHep細胞研究から見出された肝細胞分化の新規制御機構2014

    • Author(s)
      三浦静、関谷明香、鈴木淳史
    • Organizer
      第21回肝細胞研究会
    • Place of Presentation
      東京
    • Year and Date
      2014-06-27 – 2014-06-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 凝集塊形成によるiHep細胞の成熟化2014

    • Author(s)
      山本純平、関谷明香、鈴木淳史
    • Organizer
      第21回肝細胞研究会
    • Place of Presentation
      東京
    • Year and Date
      2014-06-27 – 2014-06-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] マイクロRNAによる肝芽細胞の増殖制御2014

    • Author(s)
      高島康郎、寺田茉衣子、鈴木淳史
    • Organizer
      第21回肝細胞研究会
    • Place of Presentation
      東京
    • Year and Date
      2014-06-27 – 2014-06-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 線維芽細胞から肝細胞へのダイレクトリプログラミング2014

    • Author(s)
      鈴木淳史
    • Organizer
      第66回日本細胞生物学会大会 テクニカルシンポジウム2「細胞の運命転換技術と応用」
    • Place of Presentation
      奈良
    • Year and Date
      2014-06-11 – 2014-06-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Generation of a mouse model capable of visualizing pluripotent cells in Nanog-expressing cells2014

    • Author(s)
      寺田茉衣子、関谷明香、鈴木淳史
    • Organizer
      第12回幹細胞シンポジウム
    • Place of Presentation
      福岡
    • Year and Date
      2014-05-30 – 2014-05-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] The Lin28/let-7 axis regulates proliferation of hepatoblasts2014

    • Author(s)
      高島康郎、寺田茉衣子、鈴木淳史
    • Organizer
      第12回幹細胞シンポジウム
    • Place of Presentation
      福岡
    • Year and Date
      2014-05-30 – 2014-05-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] Regulation of stem cell properties in liver development2014

    • Author(s)
      鈴木淳史
    • Organizer
      第47回日本発生生物学会大会シンポジウム「Decoding and Handling the Stem Cell System」
    • Place of Presentation
      名古屋
    • Year and Date
      2014-05-27 – 2014-05-30
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 「ダイレクトリプログラミング」、その現状と課題2014

    • Author(s)
      鈴木淳史
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] マウス線維芽細胞から肝細胞へのダイレクトリプログラミング2013

    • Author(s)
      鈴木淳史
    • Organizer
      第7回日本エピジェネティクス研究会
    • Place of Presentation
      奈良
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Direct reprogramming of fibroblasts to hepatocyte-like cells2013

    • Author(s)
      Atsushi Suzuki
    • Organizer
      23rd Conference of the Asia Pacific Association for the study of the Liver (APASL Liver Week 2013)
    • Place of Presentation
      Suntec City, Singapore
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 特定因子による皮膚細胞から肝細胞への直接転換2013

    • Author(s)
      鈴木淳史
    • Organizer
      第34回日本炎症・再生医学会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Prospective isolation and in vivo genetic lineage tracing of hepatic oval cells2013

    • Author(s)
      鈴木淳史
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Analysis of intrahepatic bile ducts using a high-resolution 3D imaging system2013

    • Author(s)
      Takashima, Y., Kawabata, M., Suzuki, A.
    • Organizer
      The 20th Annual Meeting of the Japanese Society of the Research of Hepatic Cells (JSRH)
    • Place of Presentation
      Osaka, Japan
    • Related Report
      2013 Research-status Report
  • [Presentation] Analysis of hepatic lipid metabolism using iHep cells2013

    • Author(s)
      Miura, S., Suzuki, A.
    • Organizer
      The 20th Annual Meeting of the Japanese Society of the Research of Hepatic Cells (JSRH)
    • Place of Presentation
      Osaka, Japan
    • Related Report
      2013 Research-status Report
  • [Presentation] Artificial induction and disease-related conversion of the hepatic fate2013

    • Author(s)
      Atsushi Suzuki
    • Organizer
      CSHA/ISSCR Joint Meeting on Stem Cells in Science and Medicine
    • Place of Presentation
      Suzhou, China
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Artificial induction and disease-related conversion of the hepatic fate2013

    • Author(s)
      Atsushi Suzuki
    • Organizer
      The 7th International Conference on Cell Therapy
    • Place of Presentation
      Seoul, South Korea
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] マウス線維芽細胞から肝細胞へのダイレクトリプログラミング2013

    • Author(s)
      鈴木淳史
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 線維芽細胞以外の細胞を用いた肝細胞への直接転換2013

    • Author(s)
      鵜殿美弥子、鈴木淳史
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report
  • [Presentation] iHep 細胞を用いた脂質代謝機能の解析2013

    • Author(s)
      三浦静、鈴木淳史
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report
  • [Remarks] 研究室ホームページ

    • URL

      http://www.bioreg.kyushu-u.ac.jp/labo/orgreg/top.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 研究室ホームページ

    • URL

      http://www.bioreg.kyushu-u.ac.jp/labo/orgreg/index.html

    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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