Development of the tumor marker detection method by RNA aptamer

• Project/Area Number: 25670151
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Kinki University (2015); Nagoya City University (2013-2014)
• Principal Investigator: FUJIWARA Toshinobu 近畿大学, 薬学部, 教授 (80362804)
• Research Collaborator: UENO Yoshihito 岐阜大学, 応用生物科学部, 教授 (20250467)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: RNAアプタマー / 核酸医薬 / アプタマー

Outline of Final Research Achievements

Sphingosine 1-phosphate (S1P) is combined with a sphingosine 1-phosphate receptor (S1PR) emerging in the cell surface and shows bioactivity. Five subtypes of S1PR have been identified so far, and these are tissue-specific. Recently, it has been revealed that S1PR affected the malignant transformation of cancer deeply, and the positioning as the tumor marker became important. Therefore I used high polymer material called " RNA" with the artificial ligand which could be specifically combined with each subtype of S1PR and tried that it produced wounds. However, I succeeded in the construction of the S1PR surface presentation cell and tried whole Cell SELEX, but dominant RNA aptamer was not provided.

Research Products

• [Journal Article] The molecular mechanism of translational control via the communication between the microRNA pathway and RNA-binding proteins (2015)
  • Author(s): Akira Fukao, Tomohiko Aoyama, Toshinobu Fujiwara
  • Journal Title: RNA biology Volume: 12 Issue: 9 Pages: 922-926
  • DOI: 10.1080/15476286.2015.1073436
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Regnase-1 and Roquin Regulate
a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms (2015)
  • Author(s): Takashi Mino, Yasuhiro Murakawa, Akira Fukao, Alexis Vandenbon, Hans-Hermann Wessels, Daisuke Ori, Takuya Uehata, Sarang Tartey, Shizuo Akira, Yutaka Suzuki, Carola G. Vinuesa, Uwe Ohler,
Daron M. Standley, Markus Landthaler, Toshinobu Fujiwara, Osamu Takeuchi.
  • Journal Title: Cell Volume: 161 Issue: 5 Pages: 1058-1073
  • DOI: 10.1016/j.cell.2015.04.029
  • NAID: 120005608042
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural insights into the recruitment of SMRT by the corepressor SHARP under phosphorylative regulation. (2014)
  • Author(s): Mikami S, Kanaba T, Takizawa N, Kobayashi A, Maesaki R, Fujiwara T, Ito Y,Mishima M.
  • Journal Title: Structure. Volume: 22 Issue: 1 Pages: 35-46
  • DOI: 10.1016/j.str.2013.10.007
  • Peer Reviewed
• [Journal Article] Microbial fluorescence sensing for human neurotensin receptor type lusing Ga-engineered yeast cells (2013)
  • Author(s): Jun Ishii, Asami Oda, Shota Togawa, Akira Fukao, Toshinobu Fujiwara, Chiaki Ogino and Akihiko Kondo
  • Journal Title: Analytical Biochemistry Volume: 446 Pages: 37-43
  • DOI: 10.1016/j.ab.2013.10.016
  • Peer Reviewed
• [Journal Article] The essential role for the RNA triphosphatase Cetlp in nuclear import of the mRNA capping enzyme Cetlp-Ceglp complex of Saccharomyces cerevisiae (2013)
  • Author(s): Naoki Takizawa, Toshinobu Fujiwara, Manabu Yamasaki, Ayako Saito, Akira Fukao, Akio Nomoto, Kiyobisa Mizumoto
  • Journal Title: ProsOne Volume: 8(10) Issue: 10 Pages: e78000-e78000
  • DOI: 10.1371/journal.pone.0078000
  • Peer Reviewed
• [Journal Article] Targeting cancer cell-specific RNA interference by siRNA delivery using a complex carrier of affibody-displaying bio-nanocapsules and liposomes. (2013)
  • Author(s): Nishimura Y, Mieda H, Ishii J, Ogino C, Fujiwara T, Kondo A.
  • Journal Title: J Nanobiotechnology. Volume: 11 Issue: 1 Pages: 19-19
  • DOI: 10.1186/1477-3155-11-19
  • Peer Reviewed
• [Journal Article] Structure of the second RRM domain of Nrd1, a fission yeast MAPK target RNA binding protein, and implication for its RNA recognition and regulation. (2013)
  • Author(s): Kobayashi A, Kanaba T, Satoh R, Fujiwara T, Ito Y, Sugiura R, Mishima M.
  • Journal Title: Biochem Biophys Res Commun. Volume: 437 Issue: 1 Pages: 12-17
  • DOI: 10.1016/j.bbrc.2013.06.008
  • Peer Reviewed
• [Journal Article] Staphylococcal superantigen-like protein 10 (SSL10) inhibits blood coagulation by binding to prothrombin and factor Xa via theirγ-carboxyglutamic acid (Gla) domain (2013)
  • Author(s): Itoh S, Yokoyama R, Kamoshida G, Fujiwara T, Okada H, Takii T, Tsuji T, Fujii S, Hashizume H, Onozaki K
  • Journal Title: J Biol Chem Volume: 288 Issue: 30 Pages: 21569-21580
  • DOI: 10.1074/jbc.m113.451419
  • Peer Reviewed
• [Presentation] in vitro翻訳系を用いた新規創薬ターゲット同定法 (2015)
  • Author(s): 藤原俊伸
  • Organizer: BMB2015 第38回日本分子生物学会年会 第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県）
  • Year and Date: 2015-12-01
• [Presentation] High affinity RNA for capping enzyme of Saccharomyces cerevisiae (2015)
  • Author(s): Yuka Yamada, Manabu Yamasaki, Naoki Takizawa, Akira Fukao and Toshinobu Fujiwara
  • Organizer: 11th Annual Meeting of the Oligonucleotide Therapeutics Society
  • Place of Presentation: Leiden, the Netherlands
  • Year and Date: 2015-10-11
  • Int'l Joint Research
• [Presentation] Analyses of cell type specific translation from the IRES mRNA derived from two different Poliovirus strains (2014)
  • Author(s): 貞廣暁利、藤原俊伸
  • Organizer: 第 8 回 「 長野ミーティング :生物資源の有効利用を目指して」
  • Place of Presentation: 長野
• [Presentation] Dissecting roles of Paip1 in HuD mediated translation activation (2014)
  • Author(s): 青山智彦、藤原俊伸
  • Organizer: 第 8 回 「 長野ミーティング :生物資源の有効利用を目指して」
  • Place of Presentation: 長野
• [Presentation] Effect of eIF4B phosphorylation by active Akt1 recruited into cap binding complex by HuD on translation (2014)
  • Author(s): 矢野雄暉、藤原俊伸
  • Organizer: 第 8 回 「 長野ミーティング :生物資源の有効利用を目指して」
  • Place of Presentation: 長野
• [Presentation] 高辻良文、藤原俊伸 (2014)
  • Author(s): Translational regulation by HuD through the interaction with eIF3
  • Organizer: 第 8 回 「 長野ミーティング :生物資源の有効利用を目指して」
  • Place of Presentation: 長野
• [Presentation] Functional relationship between HuD and Paip2 in differentiation of PC12 cells (2014)
  • Author(s): 大塚衆志、藤原俊伸
  • Organizer: 第 8 回 「 長野ミーティング :生物資源の有効利用を目指して」
  • Place of Presentation: 長野
• [Presentation] Novel cell free translation system using human cultured cell lines (2014)
  • Author(s): 深尾亜喜良、藤原俊伸
  • Organizer: 第 8 回 「 長野ミーティング :生物資源の有効利用を目指して」
  • Place of Presentation: 長野
• [Presentation] In vitro selection of RNA aptamer to SSTRs (2014)
  • Author(s): 山田由佳、藤原俊伸
  • Organizer: 第 8 回 「 長野ミーティング :生物資源の有効利用を目指して」
  • Place of Presentation: 長野
• [Presentation] High-throughput screening approach combining siRNA reverse-transfection and reporter mRNA transfection (2014)
  • Author(s): 藤原俊伸
  • Organizer: 第 8 回 「 長野ミーティング :生物資源の有効利用を目指して」
  • Place of Presentation: 長野
• [Presentation] The mechanism of translational control which targets a translation initiation complexa (2013)
  • Author(s): Akira Fukao, Nahum Sonenberg, Christian Thoma and Toshinobu Fujiwara
  • Organizer: 2013 RiboClub Annual Meeting
  • Place of Presentation: ケベック
• [Presentation] The RNA-binding protein HuD regulates cap-dependent translation by binding to the mRNA nuclear export receptor TAP/NXF1 (2013)
  • Author(s): Ryosuke Satoh, Akira Fukao, Akio Nomoto and Toshinobu Fujiwara
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] HuD accelerates cap-dependent translation through eIF4B phosphorylation by active Akt1 (2013)
  • Author(s): Yuki Yano, Akira Fukao and Toshinobu Fujiwara
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Remarks] 近畿大学薬学部医療薬学科生化学研究室ホームページ
  • URL: http://www.phar.kindai.ac.jp/biochemistry/index.html