Roles of Polycomb-group in links between metabolism and cancer

• Project/Area Number: 25670152
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: ISONO Kyoichi 独立行政法人理化学研究所, 統合生命医科学研究センター, 上級研究員 (90323435)
• Project Period (FY): 2013-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: エネルギー代謝 / ポリコーム群 / 遺伝子制御 / がん / 遺伝子発現制御

Research Abstract

Massive proliferation of cancer cells needs energy produced mainly by activating glycolysis. On the other hand, it is thought that elevated activities of Polycomb-group (PcG) proteins are one of causes for tumorigenesis. Understanding them will be helpful for cancer therapies. We have already identified mine metabolic kinases, which might affect PcG functions. This evidence has made us speculate interdependence between energy metabolism and PcG functions in cancer cells. In this study, we addressed this hypothesis by using gene knockdown experiments and co-immunoprecipitation assay, revealing that three of them could have something to do with PcG proteins or their functions. These results are supporting our hypothesis. We therefore believe that this project is worth being investigated continuously, contributing to applied medical science.

Research Products

• [Journal Article] Haploinsufficiency of Sf3b1 leads to compromised stem cell function but not to myelodysplasia. (2014)
  • Author(s): Matsunawa M, Yoshida K
  • Journal Title: Leukemia Volume: (Epub ahead of print) Issue: 9 Pages: 1-7
  • DOI: 10.1038/leu.2014.73
  • Peer Reviewed / Open Access
• [Journal Article] HRING1 activates Meis2 by mediating interaction of its promoter with a tissue-specific enhacer (2014)
  • Author(s): Kondo T, Isono K, Kondo K, Endo TA, Itohara S, Vidal M, Koseki
  • Journal Title: Dev Cell Volume: 28 Pages: 94-101
  • Peer Reviewed
• [Journal Article] SAM domain polymerization links subnuclear clustering of PRC1 to gene silencing (2013)
  • Author(s): Isono K, Endo AT, Ku M, Yamada D, Suzuki R, Sharif J, Ishikura T, Toyoda T, Bernstein BE, Koseki H
  • Journal Title: Dev Cell Volume: 26 Pages: 565-577
  • Peer Reviewed
• [Journal Article] WIP1, a Homeostatic Regulator of the DNA Damage Response, Is Targeted by HIPK2 for Phosphorylation and Degradation (2013)
  • Author(s): Choi DW, Na W, Kabir MH, Yi E, Kwon S, Yeom J, Ahn JW, Choi HH, Lee Y, Seo KW, Shin MK, Park SH, Yoo HY, Isono K, Koseki H, Kim ST, Lee C, Kwon YK, Choi CY
  • Journal Title: Mol Cell Volume: 51 Pages: 374-385
  • Peer Reviewed
• [Journal Article] ポリコーム群による遺伝子抑制とエピジェネティック治療への貢献 (2013)
  • Author(s): 磯野協一
  • Journal Title: 遺伝子医学MOOKエピジェネティクスと病気 Volume: 25 Pages: 37-42
• [Journal Article] SAM Domain Polymerization Links Subnuclear Clustering of PRC1 to Gene Silencing. (2013)
  • Author(s): Isono K, Endo TA, Ku M, Yamada D, Suzuki R, Sharif J, Ishikura T, Toyoda T, Bernstein BE, Koseki H.
  • Journal Title: Dev Cell. Volume: 26 Issue: 6 Pages: 565-577
  • DOI: 10.1016/j.devcel.2013.08.016
  • Peer Reviewed
• [Journal Article] ポリコーム群による遺伝子抑制とエピジェネティック治療への貢献 (2013)
  • Author(s): 磯野協一
  • Journal Title: 遺伝子医学MOOK Volume: 25 Pages: 37-42
• [Presentation] ポリコーム群Phc2リン酸化による遺伝子サイレンシング (2013)
  • Author(s): 磯野協一
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 2013-12-05
• [Presentation] 遺伝子サイレンシングにおけるポリコーム群Phc2リン酸化の役割 (2013)
  • Author(s): 磯野協一
  • Organizer: エピジェネティクス研究会
  • Place of Presentation: 奈良県新公会堂
  • Year and Date: 2013-05-30
• [Presentation] 遺伝子サイレンシングにおけるポリコーム群Phc2リン酸化の役割 (2013)
  • Author(s): 磯野協一
  • Organizer: 日本エピジェネティクス研究会
  • Place of Presentation: 奈良県新公会堂
• [Presentation] ポリコーム群Phc2リン酸化による遺伝子サイレンシング (2013)
  • Author(s): 磯野協一
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド
• [Remarks] プレスリリース 「細胞の運命を左右する新しい分子メカニズムの一端を解明」(2013年10月)
  • URL: http://www.riken.jp/pr/press/2013/20131001_1/digest/