The investigation of the role of bone in the regulation of neuron.

• Project/Area Number: 25670161
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: KOGA Takako 東京大学, 医学(系)研究科(研究院), 助教 (90451905)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 骨芽細胞 / 生理活性 / 神経細胞 / 突起伸長 / 神経突起伸長 / 細胞間制御

Outline of Final Research Achievements

The osteoblast-culture medium were confirmed to have the effect of induction of neurite elongation of PC-12 cell lines. Such putative molecules in the osteoblast-culture medium were purified and analyses by MS protein analyzation. Numerous secreted proteins were identified, but 9 of these molecules are known to be regulators of neurite outgrouth. Therefore, these 9 molecules are analyzed whethre these molecules have such effect by gain-of-function or loss-of-function experiments.