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Identification of sialyl Lewis antigen carrier proteins involved in the metastasis of lung cancer and development of diagnostic method to measure them

Research Project

Project/Area Number 25670166
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Pathological medical chemistry
Research InstitutionKochi University

Principal Investigator

HONKE Koichi  高知大学, 教育研究部医療学系, 教授 (80190263)

Co-Investigator(Kenkyū-buntansha) JIANG Songlin  高知大学, 学内共同利用施設等, 特任助教 (80598540)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords糖鎖 / シアリルルイスX / スルホルイスX / 肺腺がん / 血行性転移 / シアリルルイスX / スルホルイスX
Outline of Final Research Achievements

The sialyl Lewis X (sLeX) antigen is involved in the hematogenous metastasis of lung cancer cells. A human lung adenocarcinoma cell line ABC-1 has two sLeX carrier proteins with molecular masses of 190 kDa and more than 250 kDa. We have purified the sLeX carrier proteins using an immunoaffinity chromatography from the plasma membrane and the conditioned medium of ABC-1, and have identified protein X and Y by means of mass spectrometry.
When protein X was knockdowned by siRNA, the cell surface expression level of sLeX was unchanged. This finding suggested that the sLeX carrier protein on the cell surface was protein Y. The expression inhibitory experiments of protein Y are under way.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (10 results)

All 2015 2014 2013

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 2 results) Presentation (1 results) (of which Invited: 1 results) Book (2 results)

  • [Journal Article] Each GPI-anchored protein species forms a specific lipid raft depending on its GPI attachment signal.2015

    • Author(s)
      Miyagawa-Yamaguchi A, Kotani N, Honke K
    • Journal Title

      Glycoconj. J.

      Volume: in press Issue: 7 Pages: 531-540

    • DOI

      10.1007/s10719-015-9595-5

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ganglioside GD3 enhances invasiveness of gliomas by forming a complex with platelet-derived growth factor receptor alpha and Yes.2015

    • Author(s)
      113.Ohkawa Y, Momota H, Kato A, Hashimoto N, Tsuda Y, Kotani N, Honke K, Suzumura A, Furukawa K, Ohmi Y, Natsume A, Wakabayashi T, Furukawa K
    • Journal Title

      J. Biol. Chem.

      Volume: in press

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Segregation of Lipid Rafts Revealed by the EMARS Method Using GPI-Anchored HRP Fusion Proteins2014

    • Author(s)
      Miyagawa-Yamaguchi A, Kotani N, Honke K
    • Journal Title

      Trends in Glycoscience and Glycotechnology

      Volume: 26 Issue: 149 Pages: 59-69

    • DOI

      10.4052/tigg.26.59

    • NAID

      130004841383

    • ISSN
      0915-7352, 1883-2113
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expressed Glycosylphosphatidylinositol-AnchoredHorseradish Peroxidase Identifies Co-Clustering Molecules in Individual Lipid Raft Domains2014

    • Author(s)
      Arisa Miyagawa-Yamaguchi, Norihiro Kotani, Koichi Honke
    • Journal Title

      PLOS ONE

      Volume: 9 Issue: 3 Pages: 93054-93054

    • DOI

      10.1371/journal.pone.0093054

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Loss of branched O-mannosyl glycans in astrocytes accelerates remyelination2013

    • Author(s)
      Kanekiyo K, Inamori K, Kitazume S, Sato K, Maeda J, Higuchi M, Kizuka Y, Korekane H, Matsuo I, Honke K, Taniguchi N
    • Journal Title

      J. Neurosci.

      Volume: 33(24) Issue: 24 Pages: 10037-10047

    • DOI

      10.1523/jneurosci.3137-12.2013

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Biosynthesis and biological function of sulfoglycolipids2013

    • Author(s)
      Honke K
    • Journal Title

      Proceedings of the Japan Academy, Series B

      Volume: 89 Issue: 4 Pages: 129-138

    • DOI

      10.2183/pjab.89.129

    • NAID

      130003365112

    • ISSN
      0386-2208, 1349-2896
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Efficient siRNA delivery and tumor accumulation mediated by ionically cross-linked folic acid-poly(ethylene glycol)-chitosan oligosaccharide lactate nanoparticles: For the potential targeted ovarian cancer gene therapy2013

    • Author(s)
      Li SC, Yawata T, Honke K
    • Journal Title

      E. J. Pharm. Sci

      Volume: 52 Pages: 48-61

    • DOI

      10.1016/j.ejps.2013.10.011

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] GPI-アンカー型HRP融合タンパク質がつくる脂質ラフトドメイン2013

    • Author(s)
      本家孝一、山口亜利沙、小谷典弘
    • Organizer
      第86回日本生化学会大会、シンポジウム
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2013 Research-status Report
    • Invited
  • [Book] Handbook of Glycosyltransferases and Related Genes 2nd Ed2014

    • Author(s)
      Honke K.
    • Total Pages
      36
    • Publisher
      Springer
    • Related Report
      2013 Research-status Report
  • [Book] Medical Biochemistry 4th Ed2014

    • Author(s)
      Elbein AD, Honke K
    • Total Pages
      27
    • Publisher
      Saunders Elsevier
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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