Possible existence of lysosome-like organelle within mitochondria and its role in mitochondrial quality control
| Project/Area Number |
25670169
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathological medical chemistry
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| Research Institution | National Cancer Center Japan |
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Principal Investigator |
ARAKAWA Hirofumi 独立行政法人国立がん研究センター, 研究所, 分野長 (70313088)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | リソソーム / ミトコンドリア / タンパク質分解 / オートファジー / 活性酸素種 / 酸化ストレス / 酸化タンパク質 / 品質管理機構 / ミトコンドリア品質管理 / Mieap / 品質管理 / p53 |
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| Outline of Final Research Achievements |
In the present study, by performing electron microscopic and immune-electron microscopic analyses and protenase K protection assay, we confirmed that at least four lysosomal proteins (Cathepsin-B, Cathepsin-D, LAMP1 and LAMP2) are accumulated within mitochondria without destroying the mitochondrial structure in response to hypoxia in mouse embryonic fibroblasts and human normal mammary epithelial cells. Therefore, the Mieap-induced accumulation of lysosome-like organelles within mitochondria is a hypoxic-responsive physiological function in the cell, which is conserved at least from mouse to human.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] Dependence receptor UNC5D mediates nerve growth factor depletion-induced neuroblastoma regression.2013
Author(s)
Zhu Y, Li Y, Haraguchi S, Yu M, Ohira M, Ozaki T, Nakagawa A, Ushijima T, Isogai E, Koseki H, Nakamura Y, Kong C, Mehlen P, Arakawa H, Nakagawara A.
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Journal Title
J Clin Invest.
Volume: 123
Issue: 7
Pages: 2935-47
DOI
Related Report
Peer Reviewed
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[Presentation] Frequent inactivation of the Mieap-regulated mitochondrial quality control in pancreatic and breast cancer2014
Author(s)
Hiroki Kamino, Yasuyuki Nakamura, Hitoya Sano, Ryuya Murai, Yuri Saito, Manabu Futamura, Kazuhiro Yoshida, Nobuyuki Hiraoka, Yae Kanai, Ryoji Kushima, Toyomasa Katagiri, Hirofumi Arakawa.
Organizer
AACR Annual Meeting 2014
Place of Presentation
San Diego（USA）
Year and Date
2014-04-06
Related Report
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