Establishment of genetically engineered mouse creation foundation based on a new concept
| Project/Area Number |
25670176
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HRADA Yui 九州大学, 大学院薬学研究院, 助教 (00608507)
齊藤 智 九州大学, 薬学研究科(研究院), 研究員 (50634193)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 遺伝子操作マウス / センダイウイルスベクター / RNAヘリカーゼ / 疾患モデル |
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| Outline of Final Research Achievements |
Embryonic stem cells (ES cells) are derived from the inner cell mass of blastocysts (early embryos). These cells are pluripotent, which means that they can develop into almost any type of tissue. ES cells are used for more precise modifications of the mouse genome. We have previously developed Sendai virus (SeV) vectors that replicate in the form of negative-sense single-stranded RNA in the cytoplasm of infected cells and do not go through a DNA phase. Here we report that the SeV-mediated gene transfer into murine ES cells was very efficient and stable. This technique makes it possible to insert as well as remove or modify DNA sequences. Knock-out, knock-in and conditional mutant mice can be produced with this method.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Prognostic factors related to add-on dendritic cell vaccines on patients with inoperable pancreatic cancer receiving chemotherapy: a multicenter analysis.2014
Author(s)
Kobayashi M, Shimodaira S, Nagai K, Ogasawara M, Takahashi H, Abe H, Tanii M, Okamoto M, Tsujitani S, Yusa S, Ishidao T, Kishimoto J, Shibamoto Y, Nagaya M, Yonemitsu Y.
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Journal Title
Cancer Immunology, Immunotherapy
Volume: 印刷中
Related Report
Peer Reviewed
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[Journal Article] Dendritic cell-based immunotherapy targeting synthesized peptides for advanced biliary tract cancer2013
Author(s)
Kobayashi M, Sakabe T, Abe H, Tanii M, Takahashi H, Chiba A, Yanagida E,Shibamoto Y, Ogasawara M, Tsujitani S, Koido S, Nagai K, Shimodaira S, Okamoto M,Yonemitsu Y, Suzuki N, Nagaya M; DC-vaccine study group at the Japan Society of Innovative Cell Therapy (J-SICT).
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Journal Title
J Gastrointest Surg
Volume: 17(9)
Issue: 9
Pages: 1609-17
DOI
Related Report
Peer Reviewed
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[Journal Article] Eur J Cancer2013
Author(s)
Hidenori Takahashi
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Journal Title
10.1016/j.ejca.2012.11.005
Volume: 49
Issue: 4
Pages: 852-859
DOI
Related Report
Peer Reviewed
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