| Project/Area Number |
25670177
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Nara Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KITADAI Yasuhiko 広島大学, 医歯薬学大学院, 准教授 (10304437)
CHIHARA Yoshitomo 奈良県立医科大学, 医学部, 講師 (40405395)
FUJIMOTO Yoshio 奈良県立医科大学, 医学部, 教授 (90192731)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKAKI Miyako 奈良県立医科大学, 医学部, 特任教授 (00033358)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | がん幹細胞 / 幹細胞分化 / キネティクス / がん細胞分化・脱分化 / 多光子励起蛍光顕微鏡 / 冬眠性がん幹細胞 / 増殖性がん幹細胞 / 分化がん細胞 / 幹細胞可塑性 |
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| Outline of Final Research Achievements |
Dedifferentiation or acquisition of stemness was examined in mouse cancer cells. Expression of CD133 and nucleostemin in CT26 cells showed that differentiations of dormant cancer stem (DCS) to proliferating cancer stem (PCS) and PCS to differentiated cancer cells (DCC) were found in 3.5% and 5.6%, respectively. In contrast, dedifferentiations of DCC to PCS and PCS to DCS were found in 1.7% and 1%, respectively. In in vivo observation of KT-5 cells in mouse brain showed stem-related ALP activity was disappeared in 16%, whereas appeared in 1%. These findings suggest that the dedifferentiation or acquisition of stemness might be a common phenomenon in cancer. The biological meaning and clinical importance should be examined in further studies.
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