| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Outline of Final Research Achievements |
In this study, we established a series of mouse models of lung cancer metastasis by intracardiac inoculation of lung adenocarcinoma cell lines harboring various driver mutations, such as EGFR, KRAS, ALK, and MET. We also generated subclones that has the ability to grow in suspended condition but reattach in ordinary culture, by long-term, 3-dimensional culture under low-bind culture conditions. These subclones, designated FL clones, exhibited highly metastatic potential as compared to parental cell lines. We further performed comprehensive genomic analysis of the cell lines (parental and FL cell lines) and identified metastasis-related genes that showed altered expression in FL cells vs. parental cells, and genomic regions amplified in FL cell lines. Thus, the metastatic models created in the present study would be a promising new resource to study the molecular mechanisms of lung cancer metastasis that develop under various genetic background.
|
