| Project/Area Number |
25670191
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Experimental pathology
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| Research Institution | Shinshu University |
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Principal Investigator |
NAKAYAMA Jun 信州大学, 学術研究院医学系, 教授 (10221459)
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| Co-Investigator(Kenkyū-buntansha) |
KAKUTA Shigeru 東京大学, 農学生命科学研究科, 准教授 (80345032)
TAKAMOTO Masaya 信州大学, 医学部, 特任教授 (90226928)
KAWAKUBO Masatomo 信州大学, 学術研究院医学系, 助教 (70397305)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 胃癌 / 糖鎖 / 炎症 / サイトカイン / ダブルノックアウトマウス / ゲノム編集 / 遺伝子改変マウス / IL-33シグナル / 癌 / ノックアウトマウス |
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| Outline of Final Research Achievements |
A4gnt knockout (KO) mouse is a unique model for studying gastric cancer pathogenesis. Extensive gene analysis for gastric mucosa of A4gnt KO mouse indicated possible involvement of IL-33/ST2 axis in gastric cancer development. Aim of this study is to clarify the role of IL-33/ST2 axis on pathogenesis of gastric cancer. To this end, we generated A4gnt/Il1rl1 double KO mice by crossing A4gnt KO mice and Il1rl1 KO mice that deficient in ST2, and then compared A4gnt/Il1rl1 DKO mice with A4gnt KO mice at 10 weeks of age (n = 6). Obvious difference was not found in gastric pathology. However, real-time PCR analysis revealed that expression levels of Il33, Fgf7, Ccl2, Grem1 and Arg1 in A4gnt/Il1rl1 DKO mice were significantly reduced compared to A4gnt KO mice, suggesting that IL-33/ST2 axis regulates tumor-promoting inflammation in A4gnt KO mice.
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