The development of the novel vector that eliminates ES/iPS cell-derived tumor to realize regenerative medicine
| Project/Area Number |
25670194
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Experimental pathology
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MITSUI Kaoru 鹿児島大学, 医歯学域医学系, 講師 (40324975)
IRIE Rie 鹿児島大学, 医歯学域医学系, 助教 (90381178)
IJICHI Nobuhiro 鹿児島大学, 医歯学域医学系, 助教 (80380624)
WAN Yu-chin 鹿児島大学, 医歯学総合研究科, 分担研究員 (20505143)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 再生医学 / iPS細胞 / 遺伝子治療 / ウイルスベクター / 腫瘍溶解性ウイルス / サバイビン / 腫瘍化 / ES細胞 |
|---|
| Outline of Final Research Achievements |
Incomplete abolition of tumorigenicity creates potential safety concerns in clinical human pluripotent stem cells (hPSCs)-based regenerative medicine. We found that conditionally replicating adenoviruses that specifically target cancers using multiple factors (m-CRAs), originally developed as anticancer drugs, may also be useful as novel antitumorigenic agents in hPSC-based therapy. The survivin promoter was more active in undifferentiated hPSCs than the telomerase reverse transcriptase (TERT) promoter, whereas both promoters were minimally active in differentiated ones. Accordingly, survivin-responsive m-CRA (Surv.m-CRA) killed only undifferentiated hPSCs, but not undifferentiated ones, more efficiently than TERT-responsive m-CRAs (Tert.m-CRA). Pre-infection of hPSCs with Surv.m-CRA or Tert.m-CRA abolished in vivo teratoma formation following hPSC implantation into mice. Thus, m-CRAs may be novel antitumorigenic agents in hPSC-based regenerative medicine.
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Report
(4 results)
Research Products
(35 results)
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[Journal Article] Intravenous Administration of Endothelial Colony-Forming Cells Overexpressing Integrin β1 Augments Angiogenesis in Ischemic Legs2016
Author(s)
Goto K., Takemura G., Takahashi T., Okada H., Kanamori H., Kawamura I., Watanabe T., Morishita K., Tsujimoto A., Miyazaki N., Ushikoshi H., Kawasaki M., Mikami A., Kosai K., Minatoguchi S.
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Journal Title
Stem Cells Transl Med
Volume: 5
Issue: 2
Pages: 218-226
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Disturbance of cardiac gene expression and cardiomyocyte structure predisposes Mecp2-null mice to arrhythmias.2015
Author(s)
Hara M, Takahashi T, Mistumasu C, Igata S, Takano M, Minami T, Yasukawa H, Okayama S, Nakamura K, Okabe Y, Tanaka E, Takemura G, Kosai K, Yamashita Y, Matsuishi T.
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Journal Title
Sci Rep
Volume: 5
Issue: 1
Pages: 11204-11204
DOI
Related Report
Peer Reviewed / Open Access
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