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Analysis of KLF5/4 complex in colon cancer development

Research Project

Project/Area Number 25670197
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Experimental pathology
Research InstitutionTokyo Medical University

Principal Investigator

KURODA Masahiko  東京医科大学, 医学部, 教授 (80251304)

Co-Investigator(Kenkyū-buntansha) NAKAYA Takeo  東京医科大学, 医学部, 助教 (80512277)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsKLF5 / KLF4 / colon cancer / stem cell / 大腸がん / がん幹細胞 / 大腸癌 / 癌幹細胞
Outline of Final Research Achievements

Recently, we found that KLF5 was indispensable factor to the control of the normal intestinal stem cell and colon cancer development in vivo. To elucidate the detailed molecular mechanism of KLF4/KLF5 protein-DNA complex, we need in vivo system. However, up to now, we only have in vitro cultured cell system from a technical problem. In this project, we have succeeded development of the knock in mouse of KLF5 and KLF4 which expressed the protein with the FLAG-HA tag. We think thees mice is strong tool for analysis of physiological functions of KLF4/KLF5 protein-DNA complex in vivo.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (43 results)

All 2015 2014 2013 Other

All Journal Article (16 results) (of which Peer Reviewed: 16 results,  Open Access: 4 results,  Acknowledgement Compliant: 6 results) Presentation (24 results) (of which Invited: 7 results) Book (3 results)

  • [Journal Article] Ovarian small cell carcinoma of pulmonary type appearing in ante-mortem ascites: An autopsy case and review of the literature2015

    • Author(s)
      Hirotsugu Hashimoto, Atsushi Kurata, Koji Fujita, Hideto Shimada, Takeshi Nagai, Hajime Horiuchi, Masahiko Kuroda
    • Journal Title

      Human Pathology: Case Reports

      Volume: 未定 Issue: 3 Pages: 6-6

    • DOI

      10.1016/j.ehpc.2015.02.003

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Angiopoietin-like 7 is an anti-angiogenic protein required to prevent vascularization of the cornea.2015

    • Author(s)
      Toyono T, Usui T, Yokoo S, Taketani Y, Nakagawa S, Kuroda M, Yamagami S, Amano S.
    • Journal Title

      PLoS One

      Volume: 10 Issue: 1 Pages: e0116838-e0116838

    • DOI

      10.1371/journal.pone.0116838

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Inhibition of choroidal fibrovascular membrane formation by new class of RNA interference therapeutic agent targeting periostin.2015

    • Author(s)
      Nakama T, Yoshida S, Ishikawa K, Kobayashi Y, Zhou Y, Nakao S, Sassa Y, Oshima Y, Takao K, Shimahara A, Yoshikawa K, Hamasaki T, Ohgi T, Hayashi H, Matsuda A, Kudo A, Nozaki M, Ogura Y, Kuroda M, Ishibashi T.
    • Journal Title

      Gene Therapy.

      Volume: 22 Issue: 2 Pages: 127-137

    • DOI

      10.1038/gt.2014.112

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Immaturity of smooth muscle cells in the neointima is associated with acute coronary syndrome.2015

    • Author(s)
      Horita A, Kurata A, Ohno S, Shimoyamada H, Saito I, Kamma H, Kuroda M.
    • Journal Title

      Cardiovasc Pathol

      Volume: 24 Issue: 1 Pages: 26-32

    • DOI

      10.1016/j.carpath.2014.09.003

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evaluation of the Human Papillomavirus mRNA Test for the detection of cervical lesions in Japan.2015

    • Author(s)
      Nakayama Y, Yamada M, Kurata A, Kiseki H, Kuroda M.
    • Journal Title

      Eur J Gynaec Oncol

      Volume: in press

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Unique expression pattern of viral proteins in human herpesvirus 8-positive plasmablastic lymphoma: a case report.2014

    • Author(s)
      Yamada M, Katano H, Yotsumoto M, Hashimoto H, Muramatsu T, Shiotsuka M, Fukutake K, Kuroda M.
    • Journal Title

      Int J Clin Exp Pathol.

      Volume: 7 Pages: 6415-6418

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] In vivo challenging of polymyxins and levofloxacin eye drop against multidrug-resistant Pseudomonas aeruginosa keratitis.2014

    • Author(s)
      Tajima K, Miyake T, Koike N, Hattori T, Kumakura S, Yamaguchi T, Matsumoto T, Fujita K, Kuroda M, Ito N, Goto H.
    • Journal Title

      J Infect Chemother.

      Volume: 20 Issue: 6 Pages: 343-349

    • DOI

      10.1016/j.jiac.2013.10.015

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multifocal Kaposiform Hemangioendothelioma in Multiple Visceral Organs: An Autopsy of 9-Day-Old Female Baby.2014

    • Author(s)
      Takeo Nakaya, Kiyoko Morita, Atsushi Kurata,Tetsuo Ushiku, Takashi Igarashi, Masahiko Kuroda, Masashi Fukayama.
    • Journal Title

      Human Pathology.

      Volume: 45 Issue: 8 Pages: 1773-1777

    • DOI

      10.1016/j.humpath.2014.03.019

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Young-age-onset pancreatoduodenal carcinoma in Shwachman-Diamond syndrome.2014

    • Author(s)
      Nakaya T, Kurata A, Hashimoto H, Nishimata S, Kashiwagi Y, Fujita K, Kawashima H, Kuroda M.
    • Journal Title

      Pathol Int

      Volume: 64 Issue: 2 Pages: 75-80

    • DOI

      10.1111/pin.12133

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] KLF5 Regulates the Integrity and Oncogenicity of Intestinal Stem Cells2014

    • Author(s)
      Nakaya, T
    • Journal Title

      Cancer Res

      Volume: 74 Issue: 10 Pages: 2882-2891

    • DOI

      10.1158/0008-5472.can-13-2574

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Activin receptor-like kinase5 inhibition suppresses mouse melanoma by ubiquitin degradation of Smad4, thereby derepressing eomesodermin in cytotoxic T lymphocytes2013

    • Author(s)
      Yoon JH, Sudo K, Kuroda M. (18 名 15 番目)
    • Journal Title

      EMBO molecular Medicine

      Volume: 5(11) Issue: 11 Pages: 1720-39

    • DOI

      10.1002/emmm.201302524

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Development of Severe Pathology in Immunized Pregnant Mice Challenged with Lethal Malaria Parasites.2013

    • Author(s)
      Shoichirou Mineo, Mamoru Niikura, Shin-Ichi Inoue, Masahiko Kuroda, and Fumie Kobayashi
    • Journal Title

      Infection and Immunity

      Volume: 81 Issue: 10 Pages: 3865-3871

    • DOI

      10.1128/iai.00749-13

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Estrogen and estrogen receptor induce matrix metalloproteinase (MMP)-26 expression in endometrial carcinomacells2013

    • Author(s)
      Nishi H, Kuroda M, Isaka K
    • Journal Title

      Oncol Rep

      Volume: 30(2) Issue: 2 Pages: 751-6

    • DOI

      10.3892/or.2013.2527

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Correlation between number of renal cysts and aortic circumferences measured using autopsy material.2013

    • Author(s)
      Kurata A, Inoue S, Ohno S, Nakatsubo R, Takahashi K, Ito T, Kawasaki K, Kokubo R, Sakai T, Ubukata J, Matsubara S, Muraoka R, Yamazaki J, Hirose T, Hojo M, Watanabe E, Kuroda M.
    • Journal Title

      Pathol Res Pract

      Volume: 209 Issue: 7 Pages: 441-447

    • DOI

      10.1016/j.prp.2013.04.012

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Classification of secondary corneal amyloidosis and involvement of lactoferrin.2013

    • Author(s)
      Kaoru Araki-sasaki, Koji Hirano, Yasuhiro Osakabe, Masahiko Kuroda, Kazuko Kitagawa, Hiroshi Mishima, Hiroto Obata, Masakazu Yamada, Naoyuki Maeda, Kohji Nishida, Shigeru Kinoshita.
    • Journal Title

      Ophthalmology

      Volume: 120 Issue: 6 Pages: 1166-1172

    • DOI

      10.1016/j.ophtha.2012.11.047

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] SMAD2 MEDIATES DENDRITIC CELL SUPPRESSION BY TGF-β Smad2 is the TGF-β receptor-regulated Smad to suppress the immunogenicity of mouse and human dendritic cells.2013

    • Author(s)
      Jeong-Hwan Yoon, Katsuko Sudo, Jin Soo Han, Min-Young Chae, Seok Hee Park, Michael Weinstein, Susumu Nakae, Tadashi Yamashita, In-Kyu Lee, Takayuki Sumida, Masahiko Kuroda, and Mizuko Mamura.
    • Journal Title

      European Journal of Immunology -2

      Volume: in press

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 浸潤性乳癌に出現するlet-7d発現細胞の検討.2015

    • Author(s)
      藤田浩司、山田正俊、高梨正勝、黒田雅彦.
    • Organizer
      第104回日本病理学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-04-30 – 2015-05-02
    • Related Report
      2014 Annual Research Report
  • [Presentation] 肺線維症に対するmiR-29b 補充療法.2015

    • Author(s)
      山田侑子、高梨正勝、須藤カツ子、上田しのぶ、黒田雅彦.
    • Organizer
      第104回日本病理学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-04-30 – 2015-05-02
    • Related Report
      2014 Annual Research Report
  • [Presentation] 胃癌細胞株の形態変化における、上皮間葉系移行に関わるマイクロRNA200cとその標的の検出2015

    • Author(s)
      倉田 厚、山田正俊、大野慎一郎、橋本浩次、藤田浩司、高梨正勝、黒田雅彦.
    • Organizer
      第104回日本病理学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-04-30 – 2015-05-02
    • Related Report
      2014 Annual Research Report
  • [Presentation] 肺がん治療を想定した副作用の少ない短鎖型新規核酸医薬の開発.2015

    • Author(s)
      大野慎一郎、板野華蓮、原田裕一郎、浅田浩太朗、老川桂生、柏迫幹絵、高梨正勝、須藤カツ子、池田徳彦、黒田雅彦.
    • Organizer
      第104回日本病理学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-04-30 – 2015-05-02
    • Related Report
      2014 Annual Research Report
  • [Presentation] microRNA 生成経路を欠損する肺がん細胞株の解析.2015

    • Author(s)
      老川桂生、浅田浩太朗、大野慎一郎、黒田雅彦.
    • Organizer
      第104回日本病理学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-04-30 – 2015-05-02
    • Related Report
      2014 Annual Research Report
  • [Presentation] Development of novel form of mimic microRNA for lung cancer therapy.2015

    • Author(s)
      Ohno S, Itano K, Harada Y, Asada K, Oikawa K, Kashiwazako M, Takanashi M, Sudo K, Ikeda N, Kuroda M.
    • Organizer
      AACR Annual Meeting 2015.
    • Place of Presentation
      Philadelphia, Pennsylvania, USA
    • Year and Date
      2015-04-18 – 2015-04-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] The development of short form of mimic microRNA for lung cancer therapy.2014

    • Author(s)
      Kuroda M.
    • Organizer
      26th EORTC-NCI-ACR Symposium.
    • Place of Presentation
      Barcelona, SPAIN
    • Year and Date
      2014-11-18 – 2014-11-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] 肺がんを効果的に抑制するMimic microRNAの新規形状の探索.2014

    • Author(s)
      大野慎一郎、板野華蓮、原田裕一郎、高梨正勝、須藤カツ子、池田徳彦、黒田雅彦.
    • Organizer
      第55回日本肺癌学会学術集会
    • Place of Presentation
      京都
    • Year and Date
      2014-11-14 – 2014-11-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] エクソソームによるドラッグデリバリーシステムの応用2014

    • Author(s)
      黒田雅彦、高梨正勝、大野慎一郎.
    • Organizer
      第6回日本RNAi研究会(JARI)、 第1回日本細胞外小胞学会(JSEV).
    • Place of Presentation
      広島
    • Year and Date
      2014-08-28 – 2014-08-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] 浸潤性乳癌組織中に認めるhsa-let-7d発現細胞の出現意義について.2014

    • Author(s)
      藤田浩司、原田裕一郎、黒田雅彦.
    • Organizer
      第11回日本病理学会カンファレンス
    • Place of Presentation
      六甲山
    • Year and Date
      2014-08-01 – 2014-08-02
    • Related Report
      2014 Annual Research Report
  • [Presentation] がんを効果的に抑制する短縮型Mimic microRNA の開発.2014

    • Author(s)
      大野慎一郎、板野華蓮、原田裕一郎、高梨正勝、須藤カツ子、黒田雅彦.
    • Organizer
      第33回分子病理学研究会
    • Place of Presentation
      宮城
    • Year and Date
      2014-07-25 – 2014-07-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Colon cancer stem cells and microRNA signature during cokon cancer development.2014

    • Author(s)
      Kuroda M.
    • Organizer
      2nd International Symposium 2014
    • Place of Presentation
      Seoul, KOREA.
    • Year and Date
      2014-07-07
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 自己由細胞由来のエクソソームによるドラッグデリバリーシステムへの応用.2014

    • Author(s)
      高梨 正勝、上田 しのぶ、須藤 カツ子、石川 章夫、黒田 雅彦.
    • Organizer
      第103回日本病理学会総会
    • Place of Presentation
      広島
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 肺がんを効果的に抑制するMimic microRNAの新規形状の探索.2014

    • Author(s)
      大野 慎一郎、板野 華蓮、原田 裕一郎、高梨 正勝、須藤 カツコ、黒田 雅彦.
    • Organizer
      第103回日本病理学会総会
    • Place of Presentation
      広島
    • Related Report
      2013 Research-status Report
  • [Presentation] 胃癌細胞株における上皮間葉移行に関与するマイクロRNA200cとその標的の検出2014

    • Author(s)
      倉田 厚、山田 正俊、橋本 浩次、藤田 浩司、大野 慎一郎、高梨 正勝、黒田 雅彦
    • Organizer
      第103回日本病理学会総会
    • Place of Presentation
      広島
    • Related Report
      2013 Research-status Report
  • [Presentation] 胃癌組織におけるPD-L1の発現検討2014

    • Author(s)
      山田 正俊、藤田 浩司、倉田 厚、黒田 雅彦
    • Organizer
      第103回日本病理学会総会
    • Place of Presentation
      広島
    • Related Report
      2013 Research-status Report
  • [Presentation] Novel RNAi agent can control HCV replication.2014

    • Author(s)
      黒田 雅彦
    • Organizer
      RNA Silencing(A9) Keystone symposia
    • Place of Presentation
      Seattle Washington, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] 酸医薬品の開発2014

    • Author(s)
      黒田 雅彦
    • Organizer
      日本がん分子標的治療学会第9回トランスレーショナルリサーチワークショップ
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 新規のlipid conjugate RNAを用いたがん治療法の開発2013

    • Author(s)
      黒田 雅彦
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] 核酸医薬品の開発のためのトランスレーショナルリサーチ2013

    • Author(s)
      黒田 雅彦
    • Organizer
      日経バイオテクオンラインプロフェッショナルセミナー
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 間葉系幹細胞とエクソソーム2013

    • Author(s)
      黒田 雅彦
    • Organizer
      間葉系幹細胞を用いた再生・細胞医療早期実用化に向けた最前線
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Exosomeによるがん特異的miRNAデリバリー法の開発.2013

    • Author(s)
      黒田 雅彦、大野慎一郎、高梨 正勝
    • Organizer
      第29回日本DDS学会学術集会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] miRNA医薬品の開発

    • Author(s)
      黒田 雅彦
    • Organizer
      第6回岐阜大学先端創薬医療シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 疾患関連miRNAを標的にした臨床医薬品の開発

    • Author(s)
      黒田 雅彦
    • Organizer
      第40回日本臓器保存生物医学会学術集会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
    • Invited
  • [Book] Drug Delivery System2014

    • Author(s)
      大野慎一郎、高梨 正勝、黒田 雅彦
    • Total Pages
      5
    • Publisher
      じほう
    • Related Report
      2013 Research-status Report
  • [Book] BIO Clinica2014

    • Author(s)
      大野慎一郎、黒田 雅彦
    • Total Pages
      4
    • Publisher
      北隆館
    • Related Report
      2013 Research-status Report
  • [Book] 細胞工学2013

    • Author(s)
      大野慎一郎、髙梨 正勝、黒田 雅彦
    • Total Pages
      3
    • Publisher
      秀潤社
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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