| Project/Area Number |
25670197
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Experimental pathology
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKAYA Takeo 東京医科大学, 医学部, 助教 (80512277)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | KLF5 / KLF4 / colon cancer / stem cell / 大腸がん / がん幹細胞 / 大腸癌 / 癌幹細胞 |
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| Outline of Final Research Achievements |
Recently, we found that KLF5 was indispensable factor to the control of the normal intestinal stem cell and colon cancer development in vivo. To elucidate the detailed molecular mechanism of KLF4/KLF5 protein-DNA complex, we need in vivo system. However, up to now, we only have in vitro cultured cell system from a technical problem. In this project, we have succeeded development of the knock in mouse of KLF5 and KLF4 which expressed the protein with the FLAG-HA tag. We think thees mice is strong tool for analysis of physiological functions of KLF4/KLF5 protein-DNA complex in vivo.
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