Analysis of KLF5/4 complex in colon cancer development
Project/Area Number |
25670197
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Experimental pathology
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Research Institution | Tokyo Medical University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
NAKAYA Takeo 東京医科大学, 医学部, 助教 (80512277)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | KLF5 / KLF4 / colon cancer / stem cell / 大腸がん / がん幹細胞 / 大腸癌 / 癌幹細胞 |
Outline of Final Research Achievements |
Recently, we found that KLF5 was indispensable factor to the control of the normal intestinal stem cell and colon cancer development in vivo. To elucidate the detailed molecular mechanism of KLF4/KLF5 protein-DNA complex, we need in vivo system. However, up to now, we only have in vitro cultured cell system from a technical problem. In this project, we have succeeded development of the knock in mouse of KLF5 and KLF4 which expressed the protein with the FLAG-HA tag. We think thees mice is strong tool for analysis of physiological functions of KLF4/KLF5 protein-DNA complex in vivo.
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Report
(3 results)
Research Products
(43 results)
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[Journal Article] Inhibition of choroidal fibrovascular membrane formation by new class of RNA interference therapeutic agent targeting periostin.2015
Author(s)
Nakama T, Yoshida S, Ishikawa K, Kobayashi Y, Zhou Y, Nakao S, Sassa Y, Oshima Y, Takao K, Shimahara A, Yoshikawa K, Hamasaki T, Ohgi T, Hayashi H, Matsuda A, Kudo A, Nozaki M, Ogura Y, Kuroda M, Ishibashi T.
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Journal Title
Gene Therapy.
Volume: 22
Issue: 2
Pages: 127-137
DOI
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Peer Reviewed / Acknowledgement Compliant
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[Journal Article] In vivo challenging of polymyxins and levofloxacin eye drop against multidrug-resistant Pseudomonas aeruginosa keratitis.2014
Author(s)
Tajima K, Miyake T, Koike N, Hattori T, Kumakura S, Yamaguchi T, Matsumoto T, Fujita K, Kuroda M, Ito N, Goto H.
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Journal Title
J Infect Chemother.
Volume: 20
Issue: 6
Pages: 343-349
DOI
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Peer Reviewed
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[Journal Article] Correlation between number of renal cysts and aortic circumferences measured using autopsy material.2013
Author(s)
Kurata A, Inoue S, Ohno S, Nakatsubo R, Takahashi K, Ito T, Kawasaki K, Kokubo R, Sakai T, Ubukata J, Matsubara S, Muraoka R, Yamazaki J, Hirose T, Hojo M, Watanabe E, Kuroda M.
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Journal Title
Pathol Res Pract
Volume: 209
Issue: 7
Pages: 441-447
DOI
Related Report
Peer Reviewed
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[Journal Article] Classification of secondary corneal amyloidosis and involvement of lactoferrin.2013
Author(s)
Kaoru Araki-sasaki, Koji Hirano, Yasuhiro Osakabe, Masahiko Kuroda, Kazuko Kitagawa, Hiroshi Mishima, Hiroto Obata, Masakazu Yamada, Naoyuki Maeda, Kohji Nishida, Shigeru Kinoshita.
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Journal Title
Ophthalmology
Volume: 120
Issue: 6
Pages: 1166-1172
DOI
Related Report
Peer Reviewed
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[Journal Article] SMAD2 MEDIATES DENDRITIC CELL SUPPRESSION BY TGF-β Smad2 is the TGF-β receptor-regulated Smad to suppress the immunogenicity of mouse and human dendritic cells.2013
Author(s)
Jeong-Hwan Yoon, Katsuko Sudo, Jin Soo Han, Min-Young Chae, Seok Hee Park, Michael Weinstein, Susumu Nakae, Tadashi Yamashita, In-Kyu Lee, Takayuki Sumida, Masahiko Kuroda, and Mizuko Mamura.
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Journal Title
European Journal of Immunology -2
Volume: in press
Related Report
Peer Reviewed
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[Presentation] Development of novel form of mimic microRNA for lung cancer therapy.2015
Author(s)
Ohno S, Itano K, Harada Y, Asada K, Oikawa K, Kashiwazako M, Takanashi M, Sudo K, Ikeda N, Kuroda M.
Organizer
AACR Annual Meeting 2015.
Place of Presentation
Philadelphia, Pennsylvania, USA
Year and Date
2015-04-18 – 2015-04-22
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[Book] 細胞工学2013
Author(s)
大野慎一郎、髙梨 正勝、黒田 雅彦
Total Pages
3
Publisher
秀潤社
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