| Project/Area Number |
25670201
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Parasitology (including sanitary zoology)
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | トキソプラズマ / 病原性因子 / 転写因子 / エフェクター機構 / ROP16 / Stat3 / ES細胞 / NFAT4 / GRA6 / トキソプラズマ原虫 |
|---|
| Outline of Final Research Achievements |
In the present study, we tested whether a Toxoplasma-derived virulence factor ROP16 is capable of sustaining the undifferentiated state of embryonic stem cells (ES cells). Furthermore, we searched novel Toxoplasma-derived factors that can potentiate the differentiation of ES cells. Then, we found that overexpression of ROP16 activeted Stat3, which is essential for the maintenance of the undifferentiaion of ES cells, more strongly than LIF. In addition, we succeeded to isolate GRA6 as a novel activator of host NFAT4.
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