• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of the in vitro maintenance system for undifferentiated stem cells using Toxoplasma ROP16

Research Project

Project/Area Number 25670201
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Parasitology (including sanitary zoology)
Research InstitutionOsaka University

Principal Investigator

YAMAMOTO Masahiro  大阪大学, 微生物病研究所, 教授 (00444521)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsトキソプラズマ / 病原性因子 / 転写因子 / エフェクター機構 / ROP16 / Stat3 / ES細胞 / NFAT4 / GRA6 / トキソプラズマ原虫
Outline of Final Research Achievements

In the present study, we tested whether a Toxoplasma-derived virulence factor ROP16 is capable of sustaining the undifferentiated state of embryonic stem cells (ES cells). Furthermore, we searched novel Toxoplasma-derived factors that can potentiate the differentiation of ES cells. Then, we found that overexpression of ROP16 activeted Stat3, which is essential for the maintenance of the undifferentiaion of ES cells, more strongly than LIF. In addition, we succeeded to isolate GRA6 as a novel activator of host NFAT4.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (11 results)

All 2014 2013 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) (of which Invited: 3 results)

  • [Journal Article] Selective and strain-specific NFAT4 activation by the Toxoplasma gondii polymorphic dense granule protein GRA6.2014

    • Author(s)
      Ma JS, Sasai M, Ohshima J, Lee Y, Bando H, Takeda K, Yamamoto M
    • Journal Title

      J Exp Med.

      Volume: 211 Pages: 2013-2032

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Guanylate binding proteins promote caspase-11-dependent pyroptosis in response to cytoplasmic LPS2014

    • Author(s)
      Pilla DM, Hagar JA, Haldar AK, Mason AK, Degrandi D, Pfeffer K, Ernst RK, Yamamoto M, Miao EA, Coers J.
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 印刷中

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.2014

    • Author(s)
      Meunier E, Dick MS, Dreier RF, Schurmann N, Broz DK, Warming S, Roose-Girma M, Bumann D, Kayagaki N, Takeda K, Yamamoto M, Broz P.
    • Journal Title

      Nature

      Volume: 印刷中

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Role of the mouse and human autophagy proteins in IFN-γ-induced cell-autonomous responses against Toxoplasma gondii.2014

    • Author(s)
      Ohshima J, Lee Y, Sasai M, Saitoh T, Ma JS, Kamiyama N, Matsuura Y, Pann-Ghill S, Hayashi M, Ebisu S, Takeda K, Akira S, Yamamoto M
    • Journal Title

      J Immunol.

      Volume: 192 Pages: 3328-3335

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] The E2-Like Conjugation Enzyme Atg3 Promotes Binding of IRG and Gbp Proteins to Chlamydia- and Toxoplasma-Containing Vacuoles and Host Resistance.2014

    • Author(s)
      Haldar AK, Piro AS, Pilla DM, Yamamoto M, Coers J.
    • Journal Title

      PLoS One.

      Volume: 9

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Innate immune response induced by baculovirus attenuates transgene expression in mammalian cells.2014

    • Author(s)
      Ono C, Ninomiya A, Yamamoto S, Abe T, Wen X, Fukuhara T, Sasai M, Yamamoto M, Saitoh T, Satoh T, Kawai T, Ishii KJ, Akira S, Okamoto T, Matsuura Y.
    • Journal Title

      J Virol.

      Volume: 88 Pages: 2157-67

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] IFN-γ依存的抗トキソプラズマ応答におけるオートファジー蛋白質の役割2014

    • Author(s)
      山本雅裕、大嶋淳、馬知秀、笹井美和、李英愛
    • Organizer
      第82回日本寄生虫学会大会
    • Place of Presentation
      松山、愛媛
    • Related Report
      2013 Research-status Report
  • [Presentation] Role of the mouse and human autophagy proteins in IFN-γ-induced cell-autonomous responses against Toxoplasma gondii2014

    • Author(s)
      Ohshima J, Lee Y, Ma JS, Sasai M, Yamamoto M
    • Organizer
      第7回寄生虫感染免疫研究会
    • Place of Presentation
      高山、岐阜
    • Related Report
      2013 Research-status Report
  • [Presentation] Selective and Strain-specific NFAT4 activation by a Toxoplasma gondii polymorphic Dense Granule Protein GRA62013

    • Author(s)
      Yamamoto M
    • Organizer
      International Symposium TCUID2013 Toward Comprehensive Understanding of Immune Dynamism
    • Place of Presentation
      吹田、大阪
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] A protozoan parasite Toxoplasma gondii manipulates host cell functions by effector molecules2013

    • Author(s)
      Yamamoto M
    • Organizer
      The XIVth International Congress of Protistology
    • Place of Presentation
      バンクーバー、カナダ
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Immunological interface between host and a protozoan parasite Toxoplasma gondii

    • Author(s)
      Yamamoto M
    • Organizer
      FORUM GRADUATE SCHOOLS INFECTION & IMMUNITY BIOLOGIE-MEDECINE
    • Place of Presentation
      ジュネーブ、スイス
    • Related Report
      2013 Research-status Report
    • Invited

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi