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Development of a de novo designed molecule to develop structure-based vaccine against anthrax

Research Project

Project/Area Number 25670206
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Bacteriology (including mycology)
Research InstitutionJapanese Foundation for Cancer Research (2015)
Hokkaido University (2013-2014)

Principal Investigator

Ohnishi Naomi  公益財団法人がん研究会, その他部局等, 研究員 (50507217)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsワクチン / 抗原 / 分子設計 / 感染症 / ワクチン抗原 / 炭疽 / 細菌 / 炭疽菌
Outline of Final Research Achievements

Anthrax is a lethal disease caused by the Gram-positive, spore-forming bacterium Bacillus anthracis. However, a safe and effective vaccine for humans is not available to the general public. A previous study showed that immunization with oligomerized protective antigen (PA) of B. anthracis neutralized B. anthracis toxity more effectively than did monomeric PA. The aim of this study was to construct a de novo designed PA antigen based on structural information on PA heptamer. We calculated characteristic parameters of surface area of the PA heptamer and constructed three fully synthetic de novo designed molecules. The designed molecule was expressed in E. coli and then purified using column chromatography. We performed structural analysis and examined the antigenicity of the designed molecule using animal model and molecular basis-approaches. Such structural engineered protein is expected to contribute to the development of new prevention and treatment for various diseases.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (17 results)

All 2015 2014 2013 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (13 results) (of which Invited: 3 results) Remarks (1 results)

  • [Journal Article] <i>Bacillus cereus</i> from the environment is genetically related to the highly pathogenic <i>B. cereus</i> in Zambia2015

    • Author(s)
      Ogawa H, Ohnuma M, Squarre D, Mweene AS, Ezaki T, Fujikura D, Ohnishi N, Thomas Y, Hang'ombe BM, Higashi H.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 77 Issue: 8 Pages: 993-995

    • DOI

      10.1292/jvms.15-0059

    • NAID

      130005094678

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A novel multiplex PCR discriminates Bacillus anthracis and its genetically related strains from other Bacillus cereus group species2015

    • Author(s)
      Ogawa H., Fujikura D., Ohnuma M., Ohnishi N., Hang'ombe B.M., Mimuro M., Ezaki T., Mweene A.S., Higashi H
    • Journal Title

      PLoS One

      Volume: 10 Issue: 3 Pages: e0122004-e0122004

    • DOI

      10.1371/journal.pone.0122004

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Genome Sequence of Bacillus anthracis outbreak strain in Zambia2014

    • Author(s)
      Ohnishi N., Maruyama F., Ogawa H., Kachi H., Yamada S., Fujikura D., Nakagawa I., Hang'ombe B.M., Thomas Y., Mweene A.S., Higashi H.
    • Journal Title

      2011. Genome Announc

      Volume: 2 Issue: 2

    • DOI

      10.1128/genomea.00116-14

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Genetic Profiles of Bacillus anthracis Indigenous to Zambia and Characterization of Bacillus Species Causing Anthrax-like Disease2015

    • Author(s)
      Naomi Ohnishi
    • Organizer
      2015JSME annual meeting and 7th JTK symposium
    • Place of Presentation
      Ibaraki
    • Year and Date
      2015-10-17
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 炭疽の制圧を目指して-Bacillus 属細菌の病態-遺伝学的連関解析2015

    • Author(s)
      大西なおみ
    • Organizer
      第23回分子寄生虫学ワークショップ・第13回分子寄生虫・マラリア研究フォーラム合同大会
    • Place of Presentation
      帯広畜産大学
    • Year and Date
      2015-08-30
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] ザンビア共和国で単離された炭疽菌の全ゲノム解析に基づくゲノム疫学の構築2015

    • Author(s)
      大西なおみ、丸山史人、大崎研、東秀明
    • Organizer
      第88回日本細菌学会学術総会
    • Place of Presentation
      長良川国際会議場
    • Year and Date
      2015-03-25 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] 炭疽の制圧を目指した疫学調査と新規ワクチンの開発2014

    • Author(s)
      大西なおみ、藤倉大輔、五十嵐学、赤松玲子、豊間根耕地、東秀明
    • Organizer
      第81回日本細菌学会北海道支部学術総会
    • Place of Presentation
      札幌医科大学
    • Year and Date
      2014-08-29 – 2014-08-30
    • Related Report
      2014 Research-status Report
  • [Presentation] 炭疽の制圧を目指した疫学調査と新規ワクチンの開発2014

    • Author(s)
      大西なおみ、藤倉大輔、五十嵐学、小川寛人、東秀明
    • Organizer
      第79回日本インターフェロン・サイトカイン学会学術集会
    • Place of Presentation
      北海道大学
    • Year and Date
      2014-06-19 – 2014-06-20
    • Related Report
      2014 Research-status Report
  • [Presentation] De novo designed molecule to develop structure-based vaccine against Anthrax2014

    • Author(s)
      Ohnishi N, Mutoh M, Fujikura D, Igarashi M, Higashi H. Mutoh M, Fujikura D, Igarashi M, Higashi H.
    • Organizer
      American Society for Microbiology 114th general meeting
    • Place of Presentation
      Boston, Massachusetts, USA
    • Year and Date
      2014-05-17 – 2014-05-20
    • Related Report
      2014 Research-status Report
  • [Presentation] De novo designed molecule to develop structure-based vaccine against Anthrax2014

    • Author(s)
      Ohnishi N, Mutoh M, Fujikura D, Igarashi M, Higashi H. Mutoh M, Fujikura D, Igarashi M, Higashi H.
    • Organizer
      American Society for Microbiology 114th general meeting
    • Place of Presentation
      Boston Convention & Exhibition Center (Boston, Massachusetts, USA)
    • Related Report
      2013 Research-status Report
  • [Presentation] 新規炭疽ワクチン抗原の分子設計2014

    • Author(s)
      大西なおみ, 武藤芽未, 五十嵐学, 藤倉大輔, 東秀明
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      船堀タワーホール(東京都)
    • Related Report
      2013 Research-status Report
  • [Presentation] タンパク質四次構造情報に基づく新規炭疽ワクチン抗原の分子設計2014

    • Author(s)
      武藤芽未, 大西なおみ, 五十嵐学, 藤倉大輔, 東秀明
    • Organizer
      第3回感染症若手フォーラム
    • Place of Presentation
      やすらぎ伊王島(長崎県)
    • Related Report
      2013 Research-status Report
  • [Presentation] Development of a Novel Anthrax Vaccine Antigen and Analysis of Molecular Pathogenicity in Bacillus anthracis Infection2014

    • Author(s)
      Fujikura D, Mutoh M, Ohnishi N, Ohnuma M, Ogawa H, Bernard MH, Aaron SM, Higashi H.
    • Organizer
      Asian-African Research Forum on Emerging and Reemerging Infections 2014
    • Place of Presentation
      仙台国際センター(宮城県)
    • Related Report
      2013 Research-status Report
  • [Presentation] De novo designed molecule to develop structure-based vaccine against Anthrax2013

    • Author(s)
      Ohnishi N, Muto M, Fujikura D, Igarashi M, Higashi H.
    • Organizer
      The 12th Awaji International Forum on infection and Immunity
    • Place of Presentation
      淡路夢舞台国際会議場(兵庫県)
    • Related Report
      2013 Research-status Report
  • [Presentation] 新規抗炭疽ワクチンの開発に向けた人工設計タンパク質の作出2013

    • Author(s)
      武藤芽未, 大西なおみ, 五十嵐学, 藤倉大輔, 東秀明
    • Organizer
      第7回細菌学若手コロッセウム
    • Place of Presentation
      広島エアポートホテル(広島県)
    • Related Report
      2013 Research-status Report
  • [Presentation] 炭疽の制圧を目指した疫学調査と新規ワクチンの開発

    • Author(s)
      大西なおみ
    • Organizer
      第2回感染症国際研究センターシンポジウム
    • Place of Presentation
      東京大学(東京都)
    • Related Report
      2013 Research-status Report
    • Invited
  • [Remarks] 北海道大学人獣共通感染症リサーチセンター感染・免疫部門

    • URL

      http://www.czc.hokudai.ac.jp/immun/

    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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