| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The purpose of the project is to identify molecules of Helicobacter pylori that are responsible for an adjuvanticity on gut-associated lymphoid tissues (GALT), and to consider the possibility as new GALT-targeting adjuvant molecules. We have performed shotgun LC-MS/MS proteomics analysis of proteins from Peyer’s patches-transmissive and non-transmissive bacterial outer membranes, and identified the candidate molecules. We have successfully selected the candidate of high importance by bioinformatics method, and produced deletion mutant strains of the factors. The in vitro and in vivo analysis of the deletion mutants revealed that the factors have potential to regulate ability of targeting Peyer’s patches.
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