Development of new targeting-type adjuvant molecule derived from bacteria
Project/Area Number |
25670209
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Bacteriology (including mycology)
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Research Institution | The University of Tokyo |
Principal Investigator |
MIMURO Hitomi 東京大学, 医科学研究所, 准教授 (80396887)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 細菌 / ヘリコバクターピロリ / ワクチン |
Outline of Final Research Achievements |
The purpose of the project is to identify molecules of Helicobacter pylori that are responsible for an adjuvanticity on gut-associated lymphoid tissues (GALT), and to consider the possibility as new GALT-targeting adjuvant molecules. We have performed shotgun LC-MS/MS proteomics analysis of proteins from Peyer’s patches-transmissive and non-transmissive bacterial outer membranes, and identified the candidate molecules. We have successfully selected the candidate of high importance by bioinformatics method, and produced deletion mutant strains of the factors. The in vitro and in vivo analysis of the deletion mutants revealed that the factors have potential to regulate ability of targeting Peyer’s patches.
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Report
(3 results)
Research Products
(5 results)
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[Journal Article] The Immune Receptor NOD1 and Kinase RIP2 Interact with Bacterial Peptidoglycan on Early Endosomes to Promote Autophagy and Inflammatory Signaling2014
Author(s)
Irving AT, Mimuro H, Kufer TA, Lo C, Wheeler R, Turner LJ, Thomas BJ, Malosse C, Gantier MP, Casillas LN, Votta BJ, Bertin J, Boneca IG, Sasakawa C, Philpott DJ, Ferrero RL, Kaparakis-Liaskos M
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Journal Title
Cell Host Microbe
Volume: 15
Issue: 5
Pages: 623-635
DOI
Related Report
Peer Reviewed
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