| Project/Area Number |
25670212
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KAMITANI Shigeki 大阪府立大学, 地域保健学域, 准教授 (60379089)
ABE Hiroyuki 大阪大学, 微生物病研究所, 助教 (00379265)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 百日咳菌 / 気管支敗血症菌 / 動物モデル |
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| Outline of Final Research Achievements |
Bordtella pertussis is known as a human-specific pathogen. In this study, we attempted to generate a mutant strain of B. pertussis that infects a variety of experimental animals, which should facilitate analyses of pathogenicity of this bacterium. For this purpose, we prepared bacterial artificial chromosomes (BACs) that could be maintained in B. pertussis by improving a B. pertussis-derived plasmid or BAC for E. coli. Both the vectors generated by these distinct strategies were found to be stably maintained in B. pertussis, but did not carry a long DNA fragment (30 kbp~), the limitation which should be overcame in the future. Our final goal is to establish animal infection models of B. pertussis that carries the plasmid harboring DNA fragments derived from B. bronchiseptica with a wide host range.
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