Development of a device for the surveillance of the mutated highly pathogenic influenza viruses which acquired human-type receptor binding specificity

• Project/Area Number: 25670219
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: Chubu University
• Principal Investigator: SUZUKI Yasuo 中部大学, 生命健康科学部, 教授 (00046278)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: インフルエンザウイルス / 高病原性鳥インフルエンザウイルス / デバイス / ヒト / 監視 / 鳥 / 変異 / シアル酸 / 病原性 / 感染 / 鳥インフルエンザウイルス / 診断 / レセプター

Outline of Final Research Achievements

We developed a Novel Immunochromatographic device for Easy-to-use detection of highly pathogenic avian Influenza viruses (H5N1) with acquired human-type receptor binding specificity. This device is non-invasive for human and animal, light (less than 5gram), small (less than 15cm) and can be completed in 30min and do not require special equipment or skills, thereby avoiding some disadvantages of current methods (ELISA) for analyzing influenza virus receptor binding specificity. We confirmed its practicability using native virus isolated in Egypt, Vietnam and Indonesia. The device developed in this study can be used for the international surveillance system for human adapted H5N1 mutants in the field.

Research Products

• [Journal Article] A novel immunochromatographic system for easy-to-use detection of group 1 avian influenza viruses with acquired human-type receptor binding specificity (2014)
  • Author(s): Watanabe Y, Ito T, Ibrahim MS, Arai Y, Hotta K, Phuong HVM, Hang NLK Mai LQ, Soda K, Yamaoka M, Poetranto ED, Wulandari L, Hiramatsu H, Daidoji T, Kubota-Koketsu R, Sriwilaijaroen N, Nakaya T, Okuno Y, Takahashi T, Suzuki T, Ito T, Hotta H, Yamashiro T, Hayashi T, Morita K, Ikuta K, Suzuki Y
  • Journal Title: Biosens Bioelectron Volume: 65 Pages: 211-219
  • DOI: 10.1016/j.bios.2014.10.036
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genetics, receptor binding property, and transmissibility in mammals of naturally isolated H9N2 Avian Influenza viruses. (2014)
  • Author(s): Xuyong Li, Jianzhong Shi, Jing Guo, Guohua Deng, Qianyi Zhang, Jinliang Wang, Xijun He, Keicheng Wang, Jiming Chen, Yuanyuan Li, Jun Fan, Huiui Kong, Chunyang Gu, Yuantao Guan, Yasuo Suzuki, Yoshihiro Kawaoka, Liling Liu, Yongping Jiang, Guobin Tian, Yanbing Li, Zhigao Bu, Hualan Chen
  • Journal Title: PLoS Pathogens Volume: 10 (11) Issue: 11 Pages: e1004508-e1004508
  • DOI: 10.1371/journal.ppat.1004508
  • Peer Reviewed / Open Access
• [Journal Article] H6 influenza viruses pose a potential threat to human health (2014)
  • Author(s): Guojun Wang, Guohua Deng, Jianzhong Shi, Weiyu Luo, Guoquan Zhang, Qianyi Zhang, Liling Liu, Yongping Jiang, Chengjun Li, Nongluk Sriwilaijaroen, Hiroaki Hiramatsu, Yasuo Suzuki, Yoshihiro Kawaoka, Hualan Chen
  • Journal Title: J. Virol. Volume: 88(8) Issue: 8 Pages: 3953-3964
  • DOI: 10.1128/jvi.03292-13
  • Peer Reviewed / Open Access
• [Journal Article] Sensitive and direct detection of receptor binding specificity of highly pathogenic avian influenza A virus in clinical samples. (2013)
  • Author(s): Tadanobu Takahashi, Tatsuya Kawakami, Takashi Mizuno, Akira Minami, Yuko Uchida, Takehiko Saito, Shigeyuki Matsui, Makoto Ogata, Taichi Usui, Nongluk Sriwilaijaroen, Hiroaki Hiramatsu, Yasuo Suzuki, Takashi Suzuki.
  • Journal Title: PLoS One Volume: 8 Issue: 10 Pages: e78125-e78125
  • DOI: 10.1371/journal.pone.0078125
  • Peer Reviewed
• [Presentation] 高病原性鳥インフルエンザウイルスのヒトヘの適応性獲得の分子基盤 (2014)
  • Author(s): 鈴木康夫
  • Organizer: 日本神経ウイルス研究会
  • Place of Presentation: アクトシティ浜松・研修交流センター（静岡県・浜松市）
  • Year and Date: 2014-06-20 – 2014-06-21
  • Invited
• [Presentation] エジプトにおける患者由来H5N1高病原性鳥インフルエンザウイルスHA遺伝子の変異解析
  • Author(s): 渡邊洋平、Madiha S. Ibrahim, 大道寺 智、荒井泰葉、平松宏明、中屋隆明、鈴木康夫、生田和良
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸国際会議場
• [Book] Methods in Molecular Biology 1200; "Lectins"/Methods in Moleculoar Biology; Molecular Basis of a Pandemic of Avian-type Influenza Virus; Ed. J. Hirabayashi (2014)
  • Author(s): Nongluk Sriwilaijaroen, Yasuo Suzuki
  • Total Pages: 613
  • Publisher: Humana Press