Further improvement of virus receptor identification system
| Project/Area Number |
25670222
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | ウイルス受容体 / シュードタイプ / 細胞傷害 / ブニヤウイルス / ウイルス / 受容体の同定 / ベクター |
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| Outline of Final Research Achievements |
I have established and improved experimental system based on expression cloning to identify virus receptors efficiently. The present project is to overcome a disadvantage of the system that the systems are inapplicable to viruses whose glycoproteins cannot work as envelope proteins of retro- or lentivirus vectors. Important in my system is use of viruses which don’t give infected cells damage.
I failed to overcome the disadvantage with VSV and JEV. However, expression of a reporter fCD2 could be done with M segment of SFTS virus being known to show no/low cell damage.
Reverse genetics for SFTS virus have been reported, therefore, a next step is to prepare recombinant SFTS virus stably expressing a reporter in a future project.
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Report
(3 results)
Research Products
(24 results)
