Grant-in-Aid for Challenging Exploratory Research
We assessed the effectiveness of a novel mucosal vaccine using recombinant human parainfluenza type 2 virus (rHPIV2) as a vaccine vector. rHPIV2 showed the expression of inserted Ag in respiratory epithelial cells. M gene eliminated rHPIV2, which is replication-incompetent, incorporated cDNA of Ag85B gene from Mycobacterium bovis (rHPIV2-Ag85B) elicited Ag85B-specific immune responses. Moreover, rHPIV2-Ag85B showed protective effects against M.tuberculosis infection. We also constructed HN protein, which is receptor molecule to infection with respiratory epithelial cell, deleted rHPIV2. These findings provide evidence for the possibility of rHPIV2 as a novel mucosal vaccine vector.
intranasal immunization Vaccine
Cardiovasc Drugs Ther.
Journal of General Virology
Volume: 94(Pt 6)
Hum Vaccin Immunother.
Attribution of KAKENHI