Generation of RORgt+ Innate lymphpid cell deficient mouse

• Project/Area Number: 25670228
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: The University of Tokyo
• Principal Investigator: SAWA Shinichiro 東京大学, 医学(系)研究科(研究院), 助教 (80611756)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 自然リンパ球 / 遺伝子工学 / 腸管免疫 / 遺伝子改変マウス / 免疫学 / 発生・分化 / 細胞・組織

Outline of Final Research Achievements

Group 3 innate lymphoid cell (ILC3) develops RORgt dependent manner and plays critical roles on maintaining mucosal barrier function. In this study, with genetic strategy to dissect T cell and ILC3 development pathway, I generated new mouse model in which ILC3 can be depleted. After double strand DNA break with CRISPR/Cas9 system, loxp franked Diphteria toxin (DTR) transgene was knocked-in to the RORgt locus. I could observe DTR expression on one of the knock-in founders. I am generating ILC3 specifically deletable mice by crossing this KI founders with T cell specific Cre mouse lines.

Research Products

• [Journal Article] Immune complexes regulate bone metabolism through FcRγ signalling (2015)
  • Author(s): Negishi-Koga T, Gober HJ, Sumiya E, Komatsu N, Okamoto K, Sawa S, Suematsu A, Suda T, Sato K, Takai T, Takayanagi H.
  • Journal Title: Nat Commun Volume: 6 Issue: 1 Pages: 6637-6637
  • DOI: 10.1038/ncomms7637
  • Peer Reviewed / Open Access
• [Journal Article] 自然リンパ球（Innate lymphoid cell)の役割 (2015)
  • Author(s): 澤新一郎
  • Journal Title: 化学と生物（日本農芸化学会誌） Volume: 53 Pages: 78-81
  • Peer Reviewed
• [Journal Article] Pathogenic conversion of Foxp3+ T cells into TH17 cells in autoimmune arthritis. (2014)
  • Author(s): Komatsu, N., Okamoto, K., Sawa, S., Nakashima, T., Oh-hora, M., Kodama, T., Tanaka, S., Bluestone, JA. and Takayanagi, H.
  • Journal Title: Nature Medicine Volume: 20 Issue: 1 Pages: 62-68
  • DOI: 10.1038/nm.3432
  • Peer Reviewed / Open Access
• [Journal Article] 自然リンパ球と腸管免疫 (2013)
  • Author(s): 澤 新一郎
  • Journal Title: 皮膚アレルギーフロンティア Volume: 11 Pages: 50-51
• [Presentation] 自然リンパ球とは (2015)
  • Author(s): 澤新一郎
  • Organizer: 第39回日本リンパ学会総会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-27
  • Invited
• [Presentation] Chasing fates of innate lymphoid cell (2013)
  • Author(s): Shinichiro SAWA
  • Organizer: New Perspectives in Basic and Applied Immunology Symposium
  • Place of Presentation: ドイツ癌研究センター（ハイデルベルグ）
  • Invited
• [Presentation] Do RORgt+ innate lymphoid cells play critocal role in the intestinal immunity? (2013)
  • Author(s): Shinichiro SAWA
  • Organizer: Kyoto T cell Conference
  • Place of Presentation: 京都大学
  • Invited
• [Book] 炎症と免疫「新種の免疫細胞 自然リンパ球」 (2015)
  • Author(s): 澤新一郎
  • Total Pages: 6
  • Publisher: 先端医学社
• [Book] 医学のあゆみ「３型自然リンパ球とは？」 (2014)
  • Author(s): 澤新一郎
  • Total Pages: 3
  • Publisher: 医歯薬出版株式会社
• [Book] 実験医学「自然リンパ球研究Up to Date」 (2014)
  • Author(s): 澤新一郎
  • Total Pages: 7
  • Publisher: 羊土社