• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Characterization of an mTORC1-independent B cell differentiation program

Research Project

Project/Area Number 25670236
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Immunology
Research InstitutionKansai Medical University

Principal Investigator

MATSUDA Satoshi  関西医科大学, 医学部, 准教授 (00286444)

Co-Investigator(Renkei-kenkyūsha) OHTANI Masashi  東邦大学, 理学部, 講師 (20383713)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
KeywordsB細胞分化 / mTORC1シグナル / 腸内細菌 / mTORC1 / IgA
Outline of Final Research Achievements

Mice with a B cell-specific deletion of Raptor (RaptorB-/- mice), an essential component of mammalian target of rapamycin complex 1 (mTORC1), had a developmental arrest at the pro-B cell stage in the bone marrow (BM) and consequently failed to produce detectable levels of IgM and IgG. However, RaptorB-/- mice still produced gut-associated IgA required for controlling gut microbiota. Unexpectedly, RaptorB-/- B cells underwent somatic hyper mutation (SHM) in the gene encoding IgA despite the fact that T follicular helper (TFH) cells were absent. Our findings reveal a gut-associated mTORC1-independent differentiation program of IgA+ B cells, which plays a crucial role in maintaining intestinal homeostasis.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (7 results)

All 2014 2013 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (4 results) Remarks (1 results)

  • [Journal Article] Pleiotropic roles of mTOR complexes in haemato-lymphopoiesis and leukemogenesis.2014

    • Author(s)
      Hoshii T, Matsuda S, Hirao A.
    • Journal Title

      J Biochem.

      Volume: 156 Issue: 2 Pages: 73-83

    • DOI

      10.1093/jb/mvu037

    • NAID

      40020155036

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Loss of mTOR complex 1 induces developmental blockage in early T-lymphopoiesis and eradicates T-cell acute lymphoblastic leukemia cells.2013

    • Author(s)
      Hoshii T, Kasada A, Hatakeyama T, Ohtani M, Tadokoro Y, Naka K, Ikenoue T, Ikawa T, Kawamoto H, Fehling HJ, Araki K, Yamamura K, Matsuda S, Hirao A
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 111 Issue: 10 Pages: 3805-3810

    • DOI

      10.1073/pnas.1320265111

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] B-lineage specific loss of mTORC1 signal causes selective production of IgA against commensal bacteria.2014

    • Author(s)
      Ohtani, M., Fujii, H., Ohara, O., Koyasu, S., Kubo, M., and Matsuda, S.
    • Organizer
      第43回日本免疫学会総会・学術集会
    • Place of Presentation
      京都国際会館(京都府京都市)
    • Year and Date
      2014-12-10 – 2014-12-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] mTORC1シグナルによるB細胞分化制御2014

    • Author(s)
      松田達志、大谷真志、小安重夫,久保允人、平尾敦
    • Organizer
      第24回日本サイトメトリー学会学術集会
    • Place of Presentation
      関西医科大学(大阪府枚方市)
    • Year and Date
      2014-06-28 – 2014-06-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] mTOR complex 1 is critical for B cell development but not IgA production.2013

    • Author(s)
      Ohatni, M., Fujii, H., Koyasu, S., Kubo, M., and Matsuda, S.
    • Organizer
      第42回日本免疫学会総会・学術集会
    • Place of Presentation
      幕張(幕張メッセ)
    • Related Report
      2013 Research-status Report
  • [Presentation] mTOR complex 1はIgHμ発現を介してB細胞の分化を制御している2013

    • Author(s)
      大谷 真志、渡辺 貴志、星居 孝之、小安 重夫、小原 收、平尾 敦、久保 允人、松田 達志
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸(神戸国際会議場他)
    • Related Report
      2013 Research-status Report
  • [Remarks] 関西医科大学附属生命医学研究所生体情報部門ホームページ

    • URL

      http://www3.kmu.ac.jp/bioinfo/index.html

    • Related Report
      2014 Annual Research Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi