Multidisciplinary modificomics to search for novel and early markers to detect hepatobiliary cancers

• Project/Area Number: 25670266
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Chiba University
• Principal Investigator: NOMURA Fumio 千葉大学, 医学(系)研究科(研究院), 教授 (80164739)
• Co-Investigator(Kenkyū-buntansha): SAWAI Setsu 千葉大学, 大学院医学研究院, 助教 (10400962) ; SATOH Mamoru 千葉大学, 医学部附属病院, 寄附研究部門教員 (20401002) ; KODERA Yoshio 北里大学, 理学部, 准教授 (60265733) ; NISHIMURA Motoi 千葉大学, 医学部附属病院, 講師 (80400969)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 蛋白質 / プロテオーム / 癌 / 臨床 / 検査医学

Outline of Final Research Achievements

By comprehensive glycoproteomics, we successfully detected and identified novel protein biomarker for excessive drinking. Alterations of this protein were not as a result of liver injury, but by excessive drinking per se. Interestingly, the changes were seen in non-responders to the conventional markers for drinking. We are planning to conduct more comprehensive analyses to detect cancer specific post-translational protein modifications including phosphorylation and ubiquitination.

Research Products

• [Journal Article] Proteome-based bacterial identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS): A revolutionary shift in clinical diagnostic microbiology. (2015)
  • Author(s): Nomura F
  • Journal Title: Biochim Biophys Acta. Volume: 1854 Issue: 6 Pages: 528-37
  • DOI: 10.1016/j.bbapap.2014.10.022
  • Peer Reviewed
• [Journal Article] Moesin is a possible target molecule for cytomegalovirus-related Guillain-Barré syndrome. (2014)
  • Author(s): Sawai S, Satoh M, Mori M, Misawa S, Sogawa K, Kazami T, Ishibashi M, Beppu M, Shibuya K, Ishige T, Sekiguchi Y, Noda K, Sato K, Matsushita K, Kodera Y, Nomura F, Kuwabara S
  • Journal Title: Neurology Volume: 83 Issue: 2 Pages: 113-117
  • DOI: 10.1212/wnl.0000000000000566
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Human apolipoprotein E resequencing by proteomic analysis and its application to serotyping. (2014)
  • Author(s): Nishimura M, Satoh M, Nishimura S, Kakinuma S, Sato K, Sawai S, Matsushita K, Kado S, Kodera Y, Nomura F.
  • Journal Title: PLoS One. Volume: - Issue: 1 Pages: e85356-e85356
  • DOI: 10.1371/journal.pone.0085356
  • Peer Reviewed / Open Access
• [Journal Article] GeLC-MSプロテオーム解析による新規飲酒マーカーの探索と検証. (2014)
  • Author(s): 曽川一幸、飯田史枝、野村文夫
  • Journal Title: アルコールと医学生物学 Volume: 33 Pages: 50-5
• [Journal Article] Increased Concentrations of Apo A-I and Apo A-II Fragments in the Serum of Patients With Hepatocellular Carcinoma by Magnetic Beads-Assisted MALDI-TOF Mass Spectrometry. (2014)
  • Author(s): Liu Y, Sogawa K, Sunaga M, Umemura H, Satoh M, Kazami T, Yoshikawa M, Tomonaga T, Yokosuka O, Nomura F.
  • Journal Title: Am J Clin Pathol. Volume: 141 Issue: 1 Pages: 52-61
  • DOI: 10.1309/ajcpblfbnap6n2un
  • Peer Reviewed
• [Journal Article] Application of quantitative proteomic analysis using tandem mass tags for discovery and identification of novel biomarkers in periodontal disease (2013)
  • Author(s): Tsuchida S, Satoh M, Kawashima Y, Sogawa K, Kado S, Sawai S, Nishimura M, Ogita M
  • Journal Title: Proteomics. Volume: 13 Pages: 2339-50
  • Peer Reviewed
• [Journal Article] Serum fibrinogen alpha C-chain 5.9 kDa fragment as a biomarker for early detection of hepatic fibrosis related to hepatitis C virus (2013)
  • Author(s): Sogawa, K., Noda, K., Umemura, H., Seimiya, M., Kuga, T., Tomonaga, T., Nishimura, M., Kanai, F., Imazeki, F., Takizawa, H., Yoneda, M., Nakajima, A., Tsutsumi, M., Yokosuka, O., Nomura, F.
  • Journal Title: Proteomics Clin Appl. Volume: 7 Pages: 424-431
• [Presentation] GeLC-MSプロテオーム解析による新規飲酒マーカーの探索と検証.
  • Author(s): 曽川一幸、野村文夫ほか
  • Organizer: 第33回アルコール医学生物学研究会学術集会
  • Place of Presentation: 高知市