| Project/Area Number |
25670267
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
IDO Eiji 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (70183176)
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| Co-Investigator(Kenkyū-buntansha) |
IBUKI Kentaro 京都大学, 大学院医学研究科, 准教授 (00273524)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | フラビウイルス / デング熱 / ウエストナイル熱 / 黄熱病 / 鑑別診断 / 黄熱 / 遺伝子検査 |
|---|
| Outline of Final Research Achievements |
Mosquitoe-borne flavivirus infections such as dengue fever, West Nile fever, and yellow fever etc. exhibit clinically similar symptoms. Besides, they are sometimes serologically cross-reactive, Therefore, it was very difficult to diagnose them only by conventinal immunological tests. This study aimed at developing a rapid diagnostic system by a single universal genetic test. By scrutinizing the whole viral genomic sequences on database, we have found that part of their viral non-structural NS5 genes coding a methyltransferase and an RNA polymerase essential for viral replication would be able to differentiate the respective flavivirus species and that actually the RT-PCR by use of a set of universal primers on the concerned NS5 region clearly can tell at least 4 different serotypes of dengue viruses.
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