Development of a rapid diagnostic system for flavivirus infections focussing on the functional domains of non-structural proteins.
| Project/Area Number |
25670267
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
IDO Eiji 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (70183176)
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| Co-Investigator(Kenkyū-buntansha) |
IBUKI Kentaro 京都大学, 大学院医学研究科, 准教授 (00273524)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | フラビウイルス / デング熱 / ウエストナイル熱 / 黄熱病 / 鑑別診断 / 黄熱 / 遺伝子検査 |
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| Outline of Final Research Achievements |
Mosquitoe-borne flavivirus infections such as dengue fever, West Nile fever, and yellow fever etc. exhibit clinically similar symptoms. Besides, they are sometimes serologically cross-reactive, Therefore, it was very difficult to diagnose them only by conventinal immunological tests. This study aimed at developing a rapid diagnostic system by a single universal genetic test. By scrutinizing the whole viral genomic sequences on database, we have found that part of their viral non-structural NS5 genes coding a methyltransferase and an RNA polymerase essential for viral replication would be able to differentiate the respective flavivirus species and that actually the RT-PCR by use of a set of universal primers on the concerned NS5 region clearly can tell at least 4 different serotypes of dengue viruses.
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Report
(3 results)
Research Products
(5 results)
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[Journal Article] Establishment of in-house quantitative real-time RT-PCR assay for HIV-1 viral load measurement: application to evaluate efficacy of ART in Ghanaian patients in an urban setting.2014
Author(s)
Barnor JS, Yamamoto N, Brandful JAM, Ampofo W, Bonney K, Bonney E, Odoom JK, Aidoo S, Alalre M, Ntim NA, Amoah YO, Ofori SB, Ndzinu J, Addo NA, Nyarko A, Ido E, Ishikawa K, Yamaoka S.
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Journal Title
J AIDS Clin Res
Volume: 5
Related Report
Peer Reviewed
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