Possible circulating permeability factors related to proteinuria in nephrotic syndrome

• Project/Area Number: 25670269
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Kanazawa University
• Principal Investigator: WADA Takashi 金沢大学, 医学系, 教授 (40334784)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 蛋白尿 / ネフローゼ症候群 / オミクス / プロテオーム / 透過性因子 / バイオマーカー

Outline of Final Research Achievements

This study was aimed to determine circulating permeability factors possibly related to proteinuria in patients with nephrotic syndrome. Permeability factors derived from circulating T and/or B cells are speculated to have a crucial role in the proteinuria of nephrotic syndrome. Circulating factors in patients with beneficial effects of reducing proteinuria by removing lymphocytes treated with lymphocytapheresis were examined using proteomic studies and bioinformatics analyses. There were several circulating factors related to the onset and relapse of nephrotic syndrome. In contrast, other circulating factors could be detected when patients achieved remission. These factors might be involved in the pathogenesis of proteinuria in patients with nephrotic syndrome.

Research Products

• [Journal Article] Revisiting inflammation in diabetic nephropathy: the role of the Nlrp3 inflammasome in glomerular resident cells. (2015)
  • Author(s): Sakai N, Wada T
  • Journal Title: Kidney Int Volume: 87 Issue: 1 Pages: 12-4
  • DOI: 10.1038/ki.2014.322
  • Peer Reviewed / Open Access
• [Journal Article] Pro-inflammatory/Th1 gene expression shift in high glucose stimulated mesangial cells and tubular epithelial cells. (2014)
  • Author(s): Iwata Y, Furuichi K, Hashimoto S, Yokota K, Yasuda H, Sakai N, Kitajima S, Toyama T, Shinozaki Y, Sagara A, Matsushima K, Kaneko S, Wada T.
  • Journal Title: Biochem Biophys Res Commun Volume: 443 Issue: 3 Pages: 969-974
  • DOI: 10.1016/j.bbrc.2013.12.072
  • Peer Reviewed / Open Access
• [Journal Article] Clinical impacts of albuminuria and glomerular filtration rate on renal, cardiovascular events, and all-cause mortality in Japanese patients with type 2 diabetes. (2014)
  • Author(s): Wada T, Haneda M, Furuichi K, Babazono T, Yokoyama H, Iseki K, Araki SI, Ninomiya T, Hara S, Suzuki Y, Iwano M, Kusano E, Moriya T, Satoh H, Nakamura H, Shimizu M, Toyama T, Hara A, Makino H; The Research Group of Diabetic Nephropathy, Ministry of Health, Labour, and Welfare of Japan
  • Journal Title: Clin Exp Nephrol Volume: - Issue: 4 Pages: 613-620
  • DOI: 10.1007/s10157-013-0879-4
  • NAID: 120005411654
  • Peer Reviewed
• [Journal Article] Kidney lesions in diabetic patients with normoalbuminuric renal insufficiency. (2014)
  • Author(s): Shimizu M, Furuichi K, Yokoyama H, Toyama T, Iwata Y, Sakai N, Kaneko S, Wada T.
  • Journal Title: Clin Exp Nephrol Volume: - Issue: 2 Pages: 305-312
  • DOI: 10.1007/s10157-013-0870-0
  • Peer Reviewed
• [Journal Article] Treatment and impact of dyslipidemia in diabetic nephropathy. (2014)
  • Author(s): Toyama T, Shimizu M, Furuichi K, Kaneko S, Wada T.
  • Journal Title: Clin Exp Nephrol Volume: - Issue: 2 Pages: 201-5
  • DOI: 10.1007/s10157-013-0898-1
  • NAID: 120005349173
  • Peer Reviewed / Open Access
• [Journal Article] Impact of kidney function and urinary protein excretion on pulmonary function in Japanese patients with chronic kidney disease. (2014)
  • Author(s): Nakade Y, Toyama T, Furuichi K, Kitajima S, Ohkura N, Sagara A, Shinozaki Y, Hara A, Kitagawa K, Shimizu M, Iwata Y, Oe H, Nagahara M, Horita H, Sakai Y, Kaneko S, Wada T.
  • Journal Title: Clin Exp Nephrol Volume: - Issue: 5 Pages: 763-9
  • DOI: 10.1007/s10157-013-0920-7
  • Peer Reviewed
• [Journal Article] Coordinated Changes in DNA Methylation in Antigen-Specific Memory CD4 T Cells (2013)
  • Author(s): Hashimoto S, Ogoshi K, Sasaki A, Abe J, Qu W, Nakatani Y, Ahsan B, Oshima K, Shand FH, Ametani A, Suzuki Y, Kaneko S, Wada T, Hattori M, Sugano S,Morishita S, Matsushima K
  • Journal Title: J Immunol Volume: 190 Issue: 8 Pages: 4076-4091
  • DOI: 10.4049/jimmunol.1202267
  • Peer Reviewed
• [Journal Article] ER stress induced impaired TLR signaling and macrophage differentiation of human monocytes. (2013)
  • Author(s): Komura T, Sakai Y, Honda M, Takamura T, Wada T, Kaneko S.
  • Journal Title: Cell Immunol Volume: 282(1) Issue: 1 Pages: 44-52
  • DOI: 10.1016/j.cellimm.2013.04.006
  • Peer Reviewed
• [Journal Article] LPA1-induced cytoskeleton reorganization drives fibrosis through CTGF-dependent fibroblast proliferation. (2013)
  • Author(s): Sakai N, Chun J, Duffield JS, Wada T, Luster AD, Tager AM.
  • Journal Title: FASEB J Volume: 27 Issue: 5 Pages: 1830-1846
  • DOI: 10.1096/fj.12-219378
  • Peer Reviewed
• [Journal Article] The impacts of albuminuria and low eGFR on the risk of cardiovascular death, all-cause mortality, and renal events in diabetic patients: meta-analysis. (2013)
  • Author(s): Toyama T, Furuichi K, Ninomiya T, Shimizu M, Hara A, Iwata Y, Kaneko S, Wada T.
  • Journal Title: PLoS ONE Volume: 8 Issue: 8 Pages: e71810-e71810
  • DOI: 10.1371/journal.pone.0071810
  • NAID: 120005327232
  • Peer Reviewed
• [Journal Article] Adipose tissue-derived stem cells as a regenerative therapy for a mouse steatohepatitis-induced cirrhosis model (2013)
  • Author(s): A Seki, S Y akai, T Komura, A Nasti, K Yoshida, M Higashimoto, M Honda, S Usui, M Takamura, T Takamura, T Ochiya, K Furuichi, T Wada, S Kaneko
  • Journal Title: Hepatology Volume: 58(3) Issue: 3 Pages: 1133-42
  • DOI: 10.1002/hep.26470
  • Peer Reviewed
• [Journal Article] Long-term outcomes of Japanese type 2 diabetic patients with biopsy-proven diabetic nephropathy. (2013)
  • Author(s): Shimizu M, Furuichi K, Toyama T, Kitajima S, Hara A, Kitagawa K, Iwata Y, Sakai N, Takamura T, Yoshimura M, Yokoyama H, Kaneko S, Wada T and Kanazawa Study Group for Renal Diseases and Hypertension.
  • Journal Title: Diabetes Care Volume: 36 Issue: 11 Pages: 3655-3662
  • DOI: 10.2337/dc13-0298
  • Peer Reviewed
• [Journal Article] CCL2/CCR2 augments the production of transforming growth factor-beta1, type 1 collagen and CCL2 by human CD45-/collagen 1-positive cells under high glucose concentrations. (2013)
  • Author(s): Hara A, Sakai N, Furuichi K, Sakai Y, Takeya M, Bucala R, Mukaida N, Takuwa Y, Matsushima K, Kaneko S, Wada T.
  • Journal Title: Clin Exp Nephrol. Volume: 17 Issue: 6 Pages: 793-804
  • DOI: 10.1007/s10157-013-0796-6
  • Peer Reviewed
• [Journal Article] Long-term observations of clinicopathological characteristics and outcome of Japanese patients with pauci-immune crescentic glomerulonephritis. (2013)
  • Author(s): Kitagawa K, Furuichi K, Shinozaki Y, Toyama T, Kitajima S, Hara A, Iwata Y, Sakai N, Kaneko S, Wada T; Kanazawa Study Group for Renal Diseases and Hypertension.
  • Journal Title: Clin Exp Nephrol Volume: 17 Issue: 6 Pages: 858-865
  • DOI: 10.1007/s10157-013-0798-4
  • Peer Reviewed
• [Journal Article] Research Group of Diabetic Nephropathy, Ministry of Health, Labour, and Welfare of Japan. Japan Diabetic Nephropathy Cohort Study : study design, methods, and implementation (2013)
  • Author(s): Furuichi K1, Shimizu M, Toyama T, Koya D, Koshino Y, Abe H, Mori K, Satoh H, Imanishi M, Iwano M, Yamauchi H, Kusano E, Fujimoto S, Suzuki Y, Okuda S, Kitagawa K, Iwata Y, Kaneko S, Nishi S, Yokoyama H, Ueda Y, Haneda M, Makino H, Wada T
  • Journal Title: Clin Exp Nephrol Volume: 17 Issue: 6 Pages: 819-826
  • DOI: 10.1007/s10157-013-0778-8
  • Peer Reviewed
• [Journal Article] Validation of a newly proposed histopathological classification in Japanese patients with anti-neutrophil cytoplasmic antibody-associated glomerulonephritis. (2013)
  • Author(s): Iwakiri T, Fujimoto S, Kitagawa K, Furuichi K, Yamahana J, Matsuura Y, Yamashita A, Uezono S, Shimao Y, Hisanaga S, Tokura T, Wada T, Kitamura K, Asada Y.
  • Journal Title: BMC Nephrol Volume: 14 Issue: 1 Pages: 125-125
  • DOI: 10.1186/1471-2369-14-125
  • Peer Reviewed
• [Journal Article] Adipose tissue derived stromal stem cell therapy in murine ConA-derived hepatitis is dependent on myeloid-lineage and CD4+ T-cell suppression. (2013)
  • Author(s): Higashimoto M, Sakai Y, Takamura M, Usui S, Nasti A, Yoshida K, Seki A, Komura T, Honda M, Wada T, Furuichi K, Ochiya T, Kaneko S.
  • Journal Title: Eur J Immunol Volume: 43 Issue: 11 Pages: 2956-68
  • DOI: 10.1002/eji.201343531
  • Peer Reviewed
• [Journal Article] Clinical findings on ANCA-associated renal vasculitis from the Japan RPGN registry obtained via a questionnaire survey. (2013)
  • Author(s): Yamagata K, Usui J, Sugiyama H, Nitta K, Wada T, Muso E, Arimura Y, Koyama A, Makino H, Matsuo S.
  • Journal Title: Clin Exp Nephrol Volume: 17 Issue: 5 Pages: 646-649
  • DOI: 10.1007/s10157-012-0740-1
  • Peer Reviewed
• [Presentation] 糖尿病性腎症の病態と病期分類 (2015)
  • Author(s): 和田隆志
  • Organizer: 第49回糖尿病学の進歩
  • Place of Presentation: 岡山コンベンションセンター（岡山）
  • Year and Date: 2015-02-21
  • Invited
• [Presentation] 臨床検査の視点からみた腎臓と生体ネットワーク (2014)
  • Author(s): 和田隆志
  • Organizer: 第63回日本医学検査学会
  • Place of Presentation: 朱鷺メッセ（新潟）
  • Year and Date: 2014-05-17
  • Invited
• [Presentation] 抗糸球体基底膜抗体型腎炎の臨床病理学的所見と予後に関する検討 (2014)
  • Author(s): 小倉央行・相良明宏・篠崎康之・北島信二・遠山直志・山端潤也・北川清樹・清水和朗・岩田恭宜・坂井宣彦・清水美保・古市賢吾・和田隆志
  • Organizer: 第58回日本リウマチ学会総会・学術集会
  • Place of Presentation: グランドプリンスホテル新高輪
• [Presentation] Outcome and its related risk factors in Japanese patients with diabetic nephropathy. (2014)
  • Author(s): Takashi Wada
  • Organizer: The 3rd Chronic Kidney Disease Frontier Meeting in Nagoya, Japan
  • Place of Presentation: The Castle Plaza hotel
  • Invited
• [Presentation] ループス腎炎におけるCSF-1と免疫抑制性骨髄由来細胞の役割 (2013)
  • Author(s): 岩田恭宜・古市賢吾・和田隆志
  • Organizer: 第57回日本リウマチ学会総会・学術集会
  • Place of Presentation: 国立京都国際会館
• [Presentation] 抗糸球体基底膜抗体型腎炎の臨床病理学的所見と予後に関する検討 (2013)
  • Author(s): 小倉央行・相良明宏・北島信治・遠山直志・北川清樹・岩田恭宜・清水美保・古市賢吾・和田隆志
  • Organizer: 第57回日本リウマチ学会総会・学術集会
  • Place of Presentation: 国立京都国際会館
• [Presentation] 高糖刺激下での培養メサンギウム細胞における炎症関連遺伝子発現 (2013)
  • Author(s): 岩田恭宜・橋本真一・横田恭宣・古市賢吾・和田隆志
  • Organizer: 第56回日本腎臓学会学術総会
  • Place of Presentation: 東京国際フォーラム
• [Presentation] 糖尿病性腎症-基礎と臨床- (2013)
  • Author(s): 和田隆志
  • Organizer: 第56回日本腎臓学会学術総会
  • Place of Presentation: 東京国際フォーラム
  • Invited
• [Presentation] 糖尿病性腎症ならびに腎硬化症の診療水準向上と重症化防止にむけた調査研究 (2013)
  • Author(s): 和田隆志
  • Organizer: 第56回日本腎臓学会学術総会
  • Place of Presentation: 東京国際フォーラム
  • Invited
• [Presentation] 臨床化学が拓く新たな慢性腎臓病の研究と診療 (2013)
  • Author(s): 和田隆志
  • Organizer: 第53回日本臨床化学会年次学術集会
  • Place of Presentation: あわぎんホール（徳島）
  • Invited
• [Presentation] 糖尿病性腎症における炎症の意義とその制御 (2013)
  • Author(s): 和田隆志
  • Organizer: 第28回日本糖尿病合併症学会
  • Place of Presentation: 旭川グランドホテル
  • Invited
• [Presentation] 糖尿病性腎症レジストリーJapan Diabetic Nephropathy Chhort Study(JDNCS)の集積と展開 (2013)
  • Author(s): 古市賢吾・舟本智章・北島信治・遠山直志・北川清樹・岩田恭宜・清水美保・和田隆志
  • Organizer: 第28回日本糖尿病合併症学会
  • Place of Presentation: 旭川グランドホテル
• [Presentation] 糖尿病性腎症と高血圧：病態と治療 (2013)
  • Author(s): 和田隆志
  • Organizer: 第43回日本腎臓学会西部学術大会
  • Place of Presentation: 松山全日空ホテル
  • Invited
• [Presentation] 関節リウマチ治療におけるバイオ製剤とアフェレシス (2013)
  • Author(s): 遠山直志・古市賢吾・和田隆志
  • Organizer: 第34回日本アフェレシス学会学術大会
  • Place of Presentation: 軽井沢プリンスホテルウエスト
• [Presentation] ネフローゼ症候群に対するLCAPの有用性 (2013)
  • Author(s): 古市賢吾・和田隆志
  • Organizer: 第34回日本アフェレシス学会学術大会
  • Place of Presentation: 軽井沢プリンスホテルウエスト
• [Presentation] Dyslipidemia and kidney injury in patients with type 2 diabetes. (2013)
  • Author(s): Tadashi Toyama, Kengo Furuichi, Miho Shimizu, Yasunori Iwata, Norihiko Sakai, Shuichi Kaneko, Takashi Wada.
  • Organizer: ISN World Congress of Nephrology 2013 Satellite Symposium "Kidney and Lipids"
  • Place of Presentation: Hilton Fukuoka Sea Hawk
• [Book] 今日の治療指針2014 (2014)
  • Author(s): 和田隆志
  • Publisher: 医学書院
• [Book] 今日の治療指針 2014 (2014)
  • Author(s): 和田隆志
  • Total Pages: 1900
  • Publisher: 医学書院
• [Book] ワンランク上の検査値の読み方・考え方 第2版 (2014)
  • Author(s): 北川清樹・和田隆志
  • Total Pages: 257
  • Publisher: 総合医学社
• [Book] 系統看護学講座 別巻 臨床検査（第7版） (2014)
  • Author(s): 和田隆志
  • Total Pages: 356
  • Publisher: 医学書院
• [Book] Annual Review 腎臓 (2014)
  • Author(s): 古市賢吾・和田隆志
  • Total Pages: 294
  • Publisher: 中外医学社
• [Book] 腎疾患・透析 最新の治療 2014-2016 (2014)
  • Author(s): 遠山直志・和田隆志
  • Total Pages: 430
  • Publisher: 南江堂
• [Book] year note TOPICS 2014-2015 4th edition (2014)
  • Author(s): 北川清樹・和田隆志
  • Total Pages: 3104
  • Publisher: メディックメディア
• [Book] year note TOPICS 2013-2014 3rd edition (2013)
  • Author(s): 北川清樹・和田隆志
  • Total Pages: 3088
  • Publisher: メディックメディア
• [Book] 内科処方実践マニュアル 使い分けとさじ加減 (2013)
  • Author(s): 岩田恭宜・和田隆志
  • Total Pages: 552
  • Publisher: 日本医学出版
• [Book] 検査診断学への展望 -臨床検査指針：測定とデータ判読のポイント- (2013)
  • Author(s): 堀田宏・和田隆志
  • Total Pages: 713
  • Publisher: 南江堂
• [Book] 腎不全治療レシピ (2013)
  • Author(s): 岩田恭宜・和田隆志
  • Total Pages: 304
  • Publisher: 医学出版
• [Book] 透析患者の検査値の読み方 改訂第3版 (2013)
  • Author(s): 北川清樹・和田隆志
  • Total Pages: 460
  • Publisher: 日本メディカルセンター
• [Book] 透析患者の検査値の読み方 改訂第3版 (2013)
  • Author(s): 古市賢吾・和田隆志
  • Total Pages: 460
  • Publisher: 日本メディカルセンター
• [Book] 透析患者の検査値の読み方 改訂第3版 (2013)
  • Author(s): 北島信治・和田隆志
  • Total Pages: 460
  • Publisher: 日本メディカルセンター
• [Book] 認定医・専門医のための輸液・電解質・酸塩基平衡 (2013)
  • Author(s): 北川清樹・和田隆志
  • Total Pages: 256
  • Publisher: 中山書店
• [Book] 内科学 第10版 (2013)
  • Author(s): 和田隆志
  • Total Pages: 2872
  • Publisher: 朝倉書店
• [Book] 最新医学別冊 新しい診断と治療のABC80 ネフローゼ症候群 (2013)
  • Author(s): 山端潤也・古市賢吾・和田隆志
  • Total Pages: 268
  • Publisher: 最新医学社
• [Patent(Industrial Property Rights)] 急性腎障害の予後予測方法 (2013)
  • Inventor(s): 古市賢吾、和田隆志
  • Industrial Property Rights Holder: 金沢大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-06-06