| Budget Amount *help |
¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Outline of Final Research Achievements |
In the analysis using Escherichia coli strains derived from bacteremia, urinary tract infection, healthy subjects classified by clinical analysis, 9 pathogenic genes （fimH, afa, iha, cvaC, kpsMT, ompT, aer, usp, ibeA）were predominantly detected in strains derived from bacteremia. In the search of novel pathogenic genes, the analysis of whole genome sequence of E. coli strain derived from neonatal meningitis, homology search against BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi) and Pfam (http://pfam. sanger.ac.uk/) database were performed. As a result, 12 genes were found in which protein functions are unknown and similar structural motifs are not recognized in known proteins. Furthermore, these genes showed almost no possibility in 88 E. coli strains derived from bacteremia, urinary tract infection, and healthy individuals. From these results, we suggested that 12 unknown function genes have an important role in the passage of the blood brain barrier of E. coli.
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