Development of Diagnostics for Sarcopenia with Secretary Neurosynaptic markers

• Project/Area Number: 25670281
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Tokyo Metropolitan Institute of Medical Science
• Principal Investigator: SHIBASKI Futoshi 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 参事研究員 (90300954)
• Co-Investigator(Renkei-kenkyūsha): KAJIWARA Naoki 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主任研究員 (70453917) ; NOMURA Namiko 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野・主任基盤技術研究, 職員 (50599694)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 筋萎縮 / サルコペニア / 診断 / 加齢性筋萎縮症 / 高感度測定 / イムノPCR / 骨格筋 / バイオマーカー / 加齢 / 神経筋疾患 / リハビリ

Outline of Final Research Achievements

The early diagnosis for Sarcopenia is an urgent need for preventing irreversible muscle atrophy and for proper treatment. We succeeded in developing high sensitive multiple simultaneous Tag (MUSTag) method using specific biomarker (Protein A) for sarcopenia. In this assay, the sensitivity was calculated as 16 pg/mL (R2≧0.95) and 0.6 pg/mL (3×S.D.) in the healthy volunteer, the range is less than 300 pg/ml, but the values were measured from 500-4,800 pg/ml in patients of sarcopenia.
These results suggested the following possibilities; (1) the concentration of serum protein A shows the tendency of increase in the condition in which anabolic metabolism is promoted in the normal muscle volume. (2) in the contrast, the concentration does the tendency of decrease in the condition in which catabolic metabolism is promoted in such as inflammation and enhancement of the metabolism.

Research Products

• [Journal Article] Isolation of recombinant phage antibodies targeting the hemagglutinin cleavage site of highly pathogenic avian influenza virus (2013)
  • Author(s): Jinhua Dong, Akira Sakurai, Namiko Nomura, Enoch Y.Park, Futoshi Shibasaki, Hiroshi Ueda
  • Journal Title: PLoS One Volume: 8巻 Issue: 4 Pages: e61158-e61158
  • DOI: 10.1371/journal.pone.0061158
  • Peer Reviewed
• [Journal Article] Int6/eIF3e silenced HIF2alpha stabilization enhances migration and tube formation of HUVECs via IL-6 and IL-8 signaling (2013)
  • Author(s): Endler, A. Chen, L. Li, Q. Uchida, K. Hashimoto, T. Lu, L. Xu, G. T. Shibasaki, F.
  • Journal Title: Cytokine Volume: 62 Issue: 1 Pages: 115-122
  • DOI: 10.1016/j.cyto.2013.01.021
  • Peer Reviewed
• [Journal Article] Protein-based open sandwich immuno-PCR for sensitive detection of small biomarkers (2013)
  • Author(s): Hasan, S. Dong, J. Hara, Y. Morizane, Y. Shibasaki, F. Ueda, H.
  • Journal Title: Analytical sciences Volume: 29 Pages: 871-876
  • NAID: 10031196097
  • Peer Reviewed
• [Journal Article] Cytokine marker measurement in human neuroblastoma cells with supersensitive and multiplex assay: MUSTag technology. (2013)
  • Author(s): Miyashita R1, Chen L, Morizane Y, Takeshita Y, Fukui H, Ishizaki T, Hatakeyama K, Ohseto K, Shibasaki F, Uchino H.
  • Journal Title: Acta Neurochir Suppl. Volume: 118 Pages: 317-320
  • DOI: 10.1007/978-3-7091-1434-6_62
  • ISBN: 9783709114339, 9783709114346
  • Peer Reviewed
• [Journal Article] Development of a highly sensitive immuno-PCR assay for the measurement of alpha-galactosidase A protein levels in serum and plasma (2013)
  • Author(s): Nakano, S. Morizane, Y. Makisaka, N. Suzuki, T. Togawa, T. Tsukimura, T. Kawashima, I. Sakuraba, H. Shibasaki, F.
  • Journal Title: PLoS One Volume: 8 Issue: 11 Pages: e78588-e78588
  • DOI: 10.1371/journal.pone.0078588
  • Peer Reviewed
• [Journal Article] Broad-spectrum detection of h5 subtype influenza a viruses with a new fluorescent immunochromatography system (2013)
  • Author(s): Sakurai A, Takayama K, Nomura N, Munakata T, Yamamoto N, Tamura T, Yamada J, Hashimoto M, Kuwahara K, Sakoda Y, Suda Y, Kobayashi Y, Sakaguchi N, Kida H, Kohara M, Shibasaki F
  • Journal Title: PLoS One Volume: 8 Issue: 11 Pages: e76753-e76753
  • DOI: 10.1371/journal.pone.0076753
  • Peer Reviewed
• [Journal Article] Histopathological evaluation of the diversity of cells susceptible to H5N1 virulent avian influenza virus (2013)
  • Author(s): Ogiwara H, Yasui F, Munekata K, Takagi-Kamiya A, Munakata T, Nomura N, Shibasaki F, Kuwahara K, Sakaguchi N, Sakoda Y, Kida H, Kohara M
  • Journal Title: Am. J. Pathol Volume: 184 Issue: 1 Pages: 171-183
  • DOI: 10.1016/j.ajpath.2013.10.004
  • Peer Reviewed / Open Access
• [Journal Article] Mechanisms associated with the pathogenicity of antibodies against muscle-specific kinase in myasthenia gravis. (2013)
  • Author(s): Mori S, Shigemoto K.
  • Journal Title: Autoimmun Rev Volume: (in press) Issue: 9 Pages: 912-7
  • DOI: 10.1016/j.autrev.2013.03.005
  • Peer Reviewed
• [Journal Article] Aging of muscle (2013)
  • Author(s): Shigemoto, K. Fukunaga, T. Mori, S.
  • Journal Title: Clin Calcium Volume: 13 Pages: 23-28
  • Peer Reviewed
• [Journal Article] Daily intake of Jeju groundwater improves the skin condition of the model mouse for human atopic dermatitis. (2013)
  • Author(s): Tanaka A, Jung K, Matsuda A, Jang H, Kajiwara N, Amagai Y, Oida K, Ahn G, Ohmori K, Kang KG, Matsuda H
  • Journal Title: Journal of Dermatology Volume: 40 Issue: 3 Pages: 193-200
  • DOI: 10.1111/1346-8138.12055
  • Peer Reviewed
• [Journal Article] Circadian rhythms and the effect of glucocorticoids on expression of the clock gene period1 in canine peripheral blood mononuclear cells (2013)
  • Author(s): Ohmori K, Nishikawa S, Oku K, Oida K, Amagai Y, Kajiwara N, Jung K, Matsuda A, Tanaka A, Matsuda H
  • Journal Title: Vet. J. 2013 Volume: (in press) Issue: 3 Pages: 402-407
  • DOI: 10.1016/j.tvjl.2012.10.010
  • Peer Reviewed
• [Presentation] Development of Point of Care Testing Technology in Public Private Partnership (2015)
  • Author(s): 芝崎 太
  • Organizer: メルク主催診断セミナー
  • Place of Presentation: 東京国際フォーラム（東京都千代田区）
  • Year and Date: 2015-10-07
  • Invited
• [Presentation] インフルエンザの迅速・高感度診断を可能にした蛍光イムノクロマトグラフ法の開発 (2014)
  • Author(s): 梶原直樹、櫻井陽、野村奈美子、橋本麻紗子、芝崎太
  • Organizer: Conference for BioSignal and Medicine 2014
  • Place of Presentation: 静岡県伊豆市 ラフォーレ修善寺
  • Year and Date: 2014-11-22 – 2014-11-23
• [Presentation] 都立病院バイオバンクと臨床研究プラットフォーム (2014)
  • Author(s): 大保木啓介、小川美奈、芝崎 太
  • Organizer: Conference for BioSignal and Medicine 2014
  • Place of Presentation: 静岡県伊豆市 ラフォーレ修善寺
  • Year and Date: 2014-11-22 – 2014-11-23
• [Presentation] Pathogenic role of IL-33 in murine asthma model (JP-1) (2014)
  • Author(s): Oboki K, Kajiwara N, Nakae S, Saito H, and Shibasaki F
  • Organizer: YONSEII BK21PLUS - CBSM International Joint Symposium
  • Place of Presentation: Andon , Korea
  • Year and Date: 2014-06-21 – 2014-06-22
• [Presentation] R&D of Innovative Devices for Self-Care Monitoring and OTC Diagnosis Based on Industry-Academia Cooperation (2014)
  • Author(s): Shibasaki F, Oboki. K, Nomura N, Migita T, Ogawa M, Ohishi T, Kajiwara, N, Sadato D, and Koide T.
  • Organizer: YONSEII BK21PLUS - CBSM International Joint Symposium
  • Place of Presentation: Andon , Korea
  • Year and Date: 2014-06-21 – 2014-06-22
• [Book] 筋運動ニューロンにおける老年研究 (2013)
  • Author(s): Shigemoto, K. Mori, S. Fukunaga, T. Miyazaki, T.
  • Publisher: 日本老年医学会雑誌
• [Remarks] （公財）東京都医学総合研究所 分子医療PT ホームページ
  • URL: http://www.molmed.jp/index.html
• [Remarks] （公財）東京都医学総合研究所
  • URL: http://www.igakuken.or.jp/
• [Remarks] 分子医療PT
  • URL: http://www.molmed.jp/index.html